6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester]

6-ROX se usa predominantemente para marcar nucleótidos y secuenciar ácidos nucleicos. *CAS#: 216699-36-4*

Descripción

Los isómeros únicos de ROX se prefieren cada vez más para marcar péptidos y nucleótidos porque brindan una mejor resolución en las purificaciones de HPLC que a menudo se requieren en las bioconjugaciones.

6-ROX, SE es el otro isómero único purificado de 5(6)-ROX, SE. 6-ROX se usa predominantemente para marcar nucleótidos y secuenciar ácidos nucleicos. (Ver cat. AAT-389 para 5-ROX SE)

En comparación con otras rodaminas, ROX es muy inestable. Se deben tomar precauciones para evitar el deterioro durante el almacenamiento.

CatalogoProductoPresentación
AAT-3926-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester]5 mg
AAT-3996-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester]100 mg

Importante, Solo para uso en investigación (RUO). Almacenamiento: Congelar  (< -15 °C), Minimizar la exposición a la luz

Propiedades fisicas

Peso Molecular 631.67
DisolventeDMSO

Calculadora

Preparación de la solución de stock común.
Volumen de DMSO necesario para reconstituir la masa específica de  6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4* a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM158.311 µL791.553 µL1.583 mL7.916 mL15.831 mL
5 mM31.662 µL158.311 µL316.621 µL1.583 mL3.166 mL
10 mM15.831 µL79.155 µL158.311 µL791.553 µL1.583 mL

Espectro

Abrir en Advanced Spectrum Viewer

Propiedades Espectrales

Factor de correción (280 nm)0.168
Coeficiente de extinción (cm -1 M -1)82000
Excitación (nm)578
Emisión (nm)604
Rendimiento cuántico0.941
1 Buffer acuoso (pH 7.2)

Imagen

Figura 1. Estructura quimica de 6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS 216699-36-4*

Productos Relacionados

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6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-69-8*552578900000.178
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8*578604820000.168
5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester] *CAS 209734-74-7*578604820000.168
6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] *CAS 92557-81-8*493517830000.178
6-HEX, SE [6-Carboxy-2′,4,4′,5′,7,7′-hexachlorofluorescein, succinimidyl ester] *Single Isomer*533559730000.15
6-JOE, SE [6-Carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein, succinimidyl ester] *CAS#: 113394-23-3*520545750001
6-TET, SE [6-Carboxy-2′,4,7′,7-tetrachlorofluorescein, succinimidyl ester]521542730000.13
6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*578604820000.168
6-ROX azide578604820000.168
6-ROX alkyne578604820000.168
6-ROXtra™ SE *Superior 6-ROX Replacement*578595
6-VIC, SE [6-VIC NHS ester]526543
6-NED, SE [6-NED NHS ester]545567740002
1 Buffer pH9, 2 PBS

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Referencias

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Journal: Proc Natl Acad Sci U S A (2005): 5926

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Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
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Journal: Electrophoresis (2001): 2691

Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241

Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning
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Journal: Anal Biochem (1998): 82

Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations
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Application Notes

Sunnyvale Red™ SE, a Superior Replacement for 6-ROX SE