Cyanine 3.5 monosuccinimidyl ester

Equivalente a Cy3.5® NHS ester

Descripción

Se han utilizado una variedad de tintes de cianina 3.5 (Cy3.5®) para marcar moléculas biológicas para la formación de imágenes de fluorescencia y otros análisis bioquímicos basados en fluorescencia. Se utilizan ampliamente para marcar péptidos, proteínas y oligos, etc. Los tintes Cy3.5® tienen una fluorescencia mejorada al unirse a las proteínas. El Cy3.5® éster NHS reacciona fácilmente con los grupos amino. El Cy3® éster NHS reacciona fácilmente con los grupos amino.

AAT Bioquest ofrece ésteres de colorante Cy NHS en forma de sales de trietilamonio que son más solubles en DMSO y DMF que las sales de potasio correspondientes que ofrecen otros proveedores. Las sales de trietilamonio del tinte Cy tienen la misma reactividad y dan los conjugados idénticos a las sales de potasio del tinte Cy. Cy3.5® es una marca registrada de GE Healthcare.

CatalogoProductoPresentacion
AAT-148Cyanine 3 monosuccinimidyl ester1mg

pdfSDS pdfProtocol

Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelacion (< -15 °C). Minimizar la exposición a la luz.

Propiedades Fisicas

Peso Molecular 1291.66
DisolventeDMSO

Espectro

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Propiedades Espectrales

Factor de Corrección (260 nm)0.08
Factor de Corrección (280 nm)0.178
Coeficiente de extinción (cm -1 M -1)150000
Excitación (nm)579
Emisión (nm)591
Rendimiento Cuántico 0.15

Calculadora

Preparación de la solución de stock común

Volumen de DMSO necesario para reconstituir la masa específica de Cyanine 3.5 monosuccinimidyl éster [equivalente a Cy3.5® NHS éster] a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM77.42 µL387.099 µL774.198 µL3.871 mL7.742 mL
5 mM15.484 µL77.42 µL154.84 µL774.198 µL1.548 mL
10 mM7.742 µL38.71 µL77.42 µL387.099 µL774.198 µL
Figura 1.

Estructura química del éster monosuccinimidílico de cianina 3,5 [equivalente al éster Cy3.5® NHS]

Producto Similar

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)Correction Factor (482 nm)Correction Factor (565 nm)Correction Factor (650 nm)
Cyanine 5.5 monosuccinimidyl ester [equivalent to Cy5.5® NHS ester]6837032500000.270.050.1010.00170.0470.454

Bibliografía

Single-Molecule Characterization of Cy3. 5-Cy5. 5 Dye Pair for FRET Studies of Nucleic Acids and Nucleosomes
Authors: Ghoneim, Mohamed and Musselman, Catherine A
Journal: Journal of Fluorescence (2022): 1–9

Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization
Authors: Wang, Xiaoyan and Zhang, Yu and Xue, Wei and Wang, Hong and Qiu, Xiaozhong and Liu, Zonghua
Journal: Journal of Biomaterials Applications (2017): 923–932

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao
Journal: Colloids and Surfaces B: Biointerfaces (2017)

Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics
Authors: Guo, Yuxin and Zhang, Yang and Ma, Jinyuan and Li, Qi and Li, Yang and Zhou, Xinyi and Zhao, Dan and Song, Hua and Chen, Qing and Zhu, Xuan
Journal: Journal of Controlled Release (2017)

Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction
Authors: Liu, Cuiping and Stonestrom, Aaron J and Christian, Thomas and Yong, Jeongsik and Takase, Ryuichi and Hou, Ya-Ming and Yang, Xiaolu
Journal: Journal of Biological Chemistry (2016): 10426–10436

Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules
Authors: Ngo, John T and Adams, Stephen R and Deerinck, Thomas J and Boassa, Daniela and Rodriguez-Rivera, Frances and Palida, Sakina F and Bertozzi, Carolyn R and Ellisman, Mark H and Tsien, Roger Y
Journal: Nat Chem Biol (2016): 459–465

Design, synthesis and evaluation of VEGF-siRNA/CRS as a novel vector for gene delivery
Authors: Zhao, Wen and Zhang, Yifan and Jiang, Xueyun and Cui, Chunying
Journal: Drug Design, Development and Therapy (2016): 3851

Affinity-Controlled Protein Encapsulation into Sub-30 nm Telodendrimer Nanocarriers by Multivalent and Synergistic Interactions
Authors: Wang, Xu and Shi, Changying and Zhang, Li and Bodman, Alexa and Guo, D and an , undefined and Wang, Lili and Hall, Walter A and Wilkens, Stephan and Luo, Juntao
Journal: Biomaterials (2016)

Carboxymethyl Dextran-Stabilized Polyethylenimine-Poly (epsilon-caprolactone) Nanoparticles-Mediated Modulation of MicroRNA-34a Expression via Small-Molecule Modulator for Hepatocellular Carcinoma Therapy
Authors: Deng, Xiongwei and Yin, Zhaoxia and Zhou, Zhixiang and Wang, Yihui and Zhang, Fang and Hu, Qin and Yang, Yishu and Lu, Jianqing and Wu, Yan and Sheng, Wang and others, undefined
Journal: ACS applied materials & interfaces (2016): 17068–17079

Multiplexed single-cell in situ RNA analysis by reiterative hybridization
Authors: Xiao, Lu and Guo, Jia
Journal: Analytical Methods (2015): 7290–7295

Referencias

Ver todas las 21 referencias: Citation Explorer

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Journal: Appl Phys Lett (2011): 143703

Theranostic cRGD-BioShuttle Constructs Containing Temozolomide- and Cy7 For NIR-Imaging and Therapy
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Journal: Theranostics (2011): 381

Rational approach to select small peptide molecular probes labeled with fluorescent cyanine dyes for in vivo optical imaging
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Journal: Biochemistry (2011): 2691

In vivo detection of embryonic stem cell-derived cardiovascular progenitor cells using Cy3-labeled Gadofluorine M in murine myocardium
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Quantitative proteomics by fluorescent labeling of cysteine residues using a set of two cyanine-based or three rhodamine-based dyes
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Journal: Electrophoresis (2008): 4516

Surface plasmon resonance-enhanced fluorescence implementation of a single-step competition assay: demonstration of fatty acid measurement using an anti-fatty acid monoclonal antibody and a Cy5-labeled fatty acid
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Thiazole orange and Cy3: improvement of fluorescent DNA probes with use of short range electron transfer
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Journal: J Org Chem (2008): 4263

Near-infrared fluorescence imaging of tumor integrin alpha v beta 3 expression with Cy7-labeled RGD multimers
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Cy7-Bis-dipicolylamine-zinc
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Cy7-Tetrameric arginine-glycine-aspartic acid peptide
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Application Notes (en Ingles)

A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)