Cyanine 7 bissuccinimidyl ester

Equivalent a Cy7® bisNHS ester

Descripción

Se ha utilizado una variedad de tintes de cianina 7 (Cy7®) para marcar moléculas biológicas para imágenes de fluorescencia y otros análisis bioquímicos basados en fluorescencia. Se utilizan ampliamente para marcar péptidos, proteínas y oligos, etc. Los conjugados de colorante Cy7® son uno de los fluoróforos rojos del infrarrojo cercano más comunes utilizados en aplicaciones de imágenes in vivo. El éster Cy7® NHS reacciona fácilmente con los grupos amino.

AAT Bioquest ofrece ésteres de colorante Cy NHS en forma de sales de trietilamonio que son más solubles en DMSO y DMF que las sales de potasio correspondientes que ofrecen otros proveedores. Las sales de trietilamonio del tinte Cy tienen la misma reactividad y dan los conjugados idénticos a las sales de potasio del tinte Cy. Cy7® es la marca registrada de GE Healthcare.

CatalogoProductoPresentacion
AAT-170Cyanine 7 bissuccinimidyl ester1mg

Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelacion (< -15 °C). Minimizar la exposición a la luz.

Propiedades fisicas

Peso Molecular 1064.27
DisolventeDMSO

Espectro

Abrir en Advanced Spectrum Viewer

Propiedades Espectrales

Factor de Corrección (260 nm)0.05
Factor de Corrección (280 nm)0.036
Factor de Corrección (482 nm)0.0005
Factor de Corrección (565 nm)0.0193
Factor de Corrección (650 nm)0.165
Coeficiente de extinción (cm -1 M -1)250000
Excitación (nm)756
Emisión (nm)779
Rendimiento Cuántico0.3

Calculadora

Preparación de la solución de stock común

Volumen de DMSO necesario para reconstituir la masa específica de Cyanine 7 bissuccinimidil éster [equivalente a Cy7® bisNHS éster] a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM93.961 µL469.806 µL939.611 µL4.698 mL9.396 mL
5 mM18.792 µL93.961 µL187.922 µL939.611 µL1.879 mL
10 mM9.396 µL46.981 µL93.961 µL469.806 µL939.611 µL
Figura 1.

Estructura química del éster de bissuccinimidilo de cianina 7 [equivalente al éster de bisNHS de Cy7®]

Productos Similares

NombreExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)Correction Factor (482 nm)Correction Factor (565 nm)
Cyanine 3 bissuccinimidyl ester [equivalent to Cy3® bisNHS ester]55556915000010.1510.070.073
Cyanine 5 bissuccinimidyl ester [equivalent to Cy5® bisNHS ester]65167025000010.271, 0.420.020.030.0090.09
Cyanine 7 monosuccinimidyl ester [equivalent to Cy7® NHS ester]7567792500000.30.050.0360.00050.0193

Bibliografía

Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization
Authors: Wang, Xiaoyan and Zhang, Yu and Xue, Wei and Wang, Hong and Qiu, Xiaozhong and Liu, Zonghua
Journal: Journal of Biomaterials Applications (2017): 923–932

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao
Journal: Colloids and Surfaces B: Biointerfaces (2017)

Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics
Authors: Guo, Yuxin and Zhang, Yang and Ma, Jinyuan and Li, Qi and Li, Yang and Zhou, Xinyi and Zhao, Dan and Song, Hua and Chen, Qing and Zhu, Xuan
Journal: Journal of Controlled Release (2017)

Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction
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Journal: Journal of Biological Chemistry (2016): 10426–10436

Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules
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Journal: Nat Chem Biol (2016): 459–465

Design, synthesis and evaluation of VEGF-siRNA/CRS as a novel vector for gene delivery
Authors: Zhao, Wen and Zhang, Yifan and Jiang, Xueyun and Cui, Chunying
Journal: Drug Design, Development and Therapy (2016): 3851

Affinity-Controlled Protein Encapsulation into Sub-30 nm Telodendrimer Nanocarriers by Multivalent and Synergistic Interactions
Authors: Wang, Xu and Shi, Changying and Zhang, Li and Bodman, Alexa and Guo, D and an , undefined and Wang, Lili and Hall, Walter A and Wilkens, Stephan and Luo, Juntao
Journal: Biomaterials (2016)

Carboxymethyl Dextran-Stabilized Polyethylenimine-Poly (epsilon-caprolactone) Nanoparticles-Mediated Modulation of MicroRNA-34a Expression via Small-Molecule Modulator for Hepatocellular Carcinoma Therapy
Authors: Deng, Xiongwei and Yin, Zhaoxia and Zhou, Zhixiang and Wang, Yihui and Zhang, Fang and Hu, Qin and Yang, Yishu and Lu, Jianqing and Wu, Yan and Sheng, Wang and others, undefined
Journal: ACS applied materials & interfaces (2016): 17068–17079

Multiplexed single-cell in situ RNA analysis by reiterative hybridization
Authors: Xiao, Lu and Guo, Jia
Journal: Analytical Methods (2015): 7290–7295

Determination of the active transport of fucoidan derived from okinawa mozuku across the human intestinal caco-2 cells as assessed by size-exclusion chromatography
Authors: Nagamine, Takeaki and Hayakawa, Kou and Nakazato, Kyoumi and Iha, Masahiko
Journal: Journal of Chromatography B (2015): 187–193

Referencias

Excitation of Cy5 in self-assembled lipid bilayers using optical microresonators
Authors: Freeman LM, Li S, Dayani Y, Choi HS, Malmstadt N, Armani AM.
Journal: Appl Phys Lett (2011): 143703

Theranostic cRGD-BioShuttle Constructs Containing Temozolomide- and Cy7 For NIR-Imaging and Therapy
Authors: Wiessler M, Hennrich U, Pipkorn R, Waldeck W, Cao L, Peter J, Ehemann V, Semmler W, Lammers T, Braun K.
Journal: Theranostics (2011): 381

Rational approach to select small peptide molecular probes labeled with fluorescent cyanine dyes for in vivo optical imaging
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Journal: Biochemistry (2011): 2691

In vivo detection of embryonic stem cell-derived cardiovascular progenitor cells using Cy3-labeled Gadofluorine M in murine myocardium
Authors: Adler ED, Bystrup A, Briley-Saebo KC, Mani V, Young W, Giovanonne S, Altman P, Kattman SJ, Frank JA, Weinmann HJ, Keller GM, Fayad ZA.
Journal: JACC Cardiovasc Imaging (2009): 1114

Quantitative proteomics by fluorescent labeling of cysteine residues using a set of two cyanine-based or three rhodamine-based dyes
Authors: Volke D, Hoffmann R.
Journal: Electrophoresis (2008): 4516

Surface plasmon resonance-enhanced fluorescence implementation of a single-step competition assay: demonstration of fatty acid measurement using an anti-fatty acid monoclonal antibody and a Cy5-labeled fatty acid
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Journal: Anal Biochem (2008): 243

Thiazole orange and Cy3: improvement of fluorescent DNA probes with use of short range electron transfer
Authors: Menacher F, Rubner M, Berndl S, Wagenknecht HA.
Journal: J Org Chem (2008): 4263

Near-infrared fluorescence imaging of tumor integrin alpha v beta 3 expression with Cy7-labeled RGD multimers
Authors: Wu Y, Cai W, Chen X.
Journal: Mol Imaging Biol (2006): 226

Cy7-Bis-dipicolylamine-zinc
Authors: Leung K., undefined
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)

Cy7-Tetrameric arginine-glycine-aspartic acid peptide
Authors: Cheng KT., undefined
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)

Application Notes (en Ingles)

A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)