El colorante iFluor® 610 succinimidyl ester es una excelente alternativa al colorante de etiquetado Alexa Fluor® 610
Descripción
Los tintes iFluor® de AAT Bioquest están optimizados para marcar proteínas, en particular anticuerpos. Estos tintes son brillantes, fotoestables y tienen un enfriamiento mínimo de las proteínas. Pueden excitarse bien con las principales líneas láser de los instrumentos de fluorescencia (p. ej., 350, 405, 488, 555 y 633 nm).
Los colorantes iFluor® 610 tienen un máximo de excitación y emisión de fluorescencia de ~610 nm y ~628 nm respectivamente. Estas características espectrales los convierten en un excelente reemplazo para el colorante de etiquetado Alexa Fluor® 610 (Alexa Fluor® es la marca registrada de Invitrogen).
iFluor® 610 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de proteínas.
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-1038 | iFluor® 610 succinimidyl ester | 1mg |
| AAT-71038 | iFluor® 610 succinimidyl ester | 100 ug |
| AAT-71516 | iFluor® 610 succinimidyl ester | 5mg |
| AAT-71566 | iFluor® 610 succinimidyl ester | 10mg |
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelación (< -15 °C). Minimizar la exposición a la luz.
Propiedades fisicas
| Peso Molecular | 1253.52 |
| Disolvente | DMSO |
Espectro
Abrir en Advanced Spectrum Viewer
Propiedades espectrales
| Factor de corrección (260 nm) | 0.32 |
| Factor de corrección (280 nm) | 0.49 |
| Coeficiente de extinción (cm -1 M -1) | 1100001 |
| Excitación (nm) | 610 |
| Emisión (nm) | 628 |
| Rendimiento cuántico | 0.851 |
Calculadora
Preparación de la solución de stock común
Volumen de DMSO necesario para reconstituir la masa específica de succinimidil éster iFluor® 610 a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de la muestra para conocer los buffers experimentales/fisiológicos apropiados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 79.775 µL | 398.877 µL | 797.754 µL | 3.989 mL | 7.978 mL |
| 5 mM | 15.955 µL | 79.775 µL | 159.551 µL | 797.754 µL | 1.596 mL |
| 10 mM | 7.978 µL | 39.888 µL | 79.775 µL | 398.877 µL | 797.754 µL |
Imagenes
Figura 1. Las células HeLa se tiñeron con antitubulina de ratón seguido de iFluorTM 610 de cabra anti-ratón IgG (H+L), y los núcleos se tiñeron con Nuclear GreenTM DCS1 (Cat# 17550).
Figura 2. Las células HeLa se tiñeron con (Tubulin+) o sin (Tubulin-) antitubulina de ratón y luego se visualizaron con iFluor® 610 cabra anti-ratón IgG (derecha) o Alexa Fluor® 610 cabra anti-ratón IgG (izquierda).
Figura 3. Arriba) El patrón espectral se generó usando un citómetro espectral de 4 láseres. Se utilizaron láseres desplazados espacialmente (355 nm, 405 nm, 488 nm y 640 nm) para crear cuatro perfiles de emisión distintos y luego, cuando se combinaron, produjeron la firma espectral general. Abajo) Análisis de citometría de flujo de células de sangre completa teñidas con conjugado de PE/iFluor® 610 anti-CD4 humano SK3. La señal de fluorescencia se controló utilizando un citómetro de flujo Aurora en el canal B6-A específico de PE/iFluor® 610.
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Bibliografiía
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Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
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Bibliografía
Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: Metabolomics (Los Angel) (2017): 2153–0769
Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: Proceedings of the National Academy of Sciences (2016): E2011–E2018
Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: International Journal of Cancer (2012): E425–E436
Referencias
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Application Notes
iFluor® Dye Selection Guide
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
FAQ
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