ReadiUse™ Preactivated HRP NHS éster proporciona el método más sólido para realizar la conjugación de anticuerpos con HRP.
Descripción
La maleimida preactivada ReadiUse™ es un derivado de HRP reactivo con tiol. Se puede utilizar para marcar fácilmente proteínas (como anticuerpos) u otras moléculas biológicas que tengan un grupo tiol.
ReadiUse™ Preactivated HRP maleimide es resistente y fácil de usar. Se puede usar para marcar anticuerpos con una simple mezcla, es decir, la solución ligeramente ácida o neutra de un anticuerpo (pH 6-7) se puede mezclar directamente con la maleimida HRP y la mezcla de reacción se agita durante 1-2 horas. En la mayoría de los casos, la solución resultante puede usarse directamente para ensayos ELISA sin más purificación.
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-11026 | ReadiUse™ Preactivated HRP maleimide | 300 ug |
Importante, Solo para uso en investigación (RUO). Almacenamiento: Congelación a < -15 °C. Minimizar la exposición a la luz.
Imagen
Figura 1. La maleimida HRP preactivada ReadiUse™ se puede usar para marcar proteínas que contienen tiol de la manera que se muestra. La reacción es una simple mezcla de 1 a 2 horas y, en la mayoría de los casos, no requiere más purificación antes de su uso.
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Referencias
Ver todas las 61 referencias: Citation Explorer
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A new, sensitive method for enzyme kinetic studies of scarce glucosides
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K+-independent actions of diazoxide question the role of inner membrane KATP channels in mitochondrial cytoprotective signaling
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A Role for Mitochondrial Dysfunction in Perpetuating Radiation-Induced Genomic Instability
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Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V
FAQ
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Can DAPI bind to RNA?
Can DAPI stain dead cells?
AssayWise
HRP Antibody Labeling Using Buccutite™ Crosslinking Technology
iFluor® 700 Dyes
Hydroxyl Radical Detection
Peroxidase Detection
Buccutite™ Conjugation Kits: Quick and Easy Antibody Labeling