ReadiUse™ Preactivated HRP NHS éster proporciona el método más sólido para realizar la conjugación de anticuerpos con HRP.
Descripción
ReadiUse™ Preactivated HRP NHS éster es el mono NHS éster de HRP. Se puede usar para marcar fácilmente proteínas (como anticuerpos) u otras moléculas biológicas que tienen un grupo amino.
El éster HRP NHS preactivado ReadiUse™ es robusto y fácil de usar. Se puede utilizar para marcar anticuerpos con una simple mezcla, es decir, la solución básica de un anticuerpo (pH 8,5-9,0) se puede mezclar directamente con el éster HRP NHS y la mezcla de reacción se agita durante 1-2 horas. En la mayoría de los casos, la solución resultante puede usarse directamente para ensayos ELISA sin más purificación.
ReadiUse™ Preactivated HRP NHS éster proporciona el método más sólido para realizar la conjugación de anticuerpos con HRP. Cualquier cantidad adecuada de anticuerpo (>1 mg/mL) se puede conjugar fácilmente con el éster NHS de HRP preactivado sin necesidad de purificación.
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-11025 | ReadiUse™ Preactivated HRP NHS ester | 300 ug |
Importante, Solo para uso en investigación (RUO). Almacenamiento: Congelación a < -15 °C. Minimizar la exposición a la luz.
Imagen
Figura 1. Mecanismo para la conjugación de HRP a la proteína objetivo usando éster de NHS activado.
Bibliografía
Ver todas las 12 bibliografias: Citation Explorer
Florfenicol binding to molecularly imprinted polymer nanoparticles in model and real samples
Authors: Caro, Nelson and Bruna, Tamara and Guerreiro, Antonio and Alvarez-Tejos, Paola and Garret{\’o}n, Virginia and Piletsky, Sergey and Gonz{\’a}lez-Casanova, Jorge and Rojas-G{\’o}mez, Diana and Ehrenfeld, Nicole
Journal: Nanomaterials (2020): 306
New method for immobilising diverse proteins onto cubic micro-protein polyhedrin crystals
Authors: Yuasa, Haruna and Kotani, Eiji and Mori, Hajime and Takaki, Keiko
Journal: Protein expression and purification (2019): 105531
Assessment of Tofacitinib and Ruxolitinib and their Anti Inflammatory Effects on Myeloperoxidase
Authors: Milton, Amber
Journal: (2017)
Patterned Photonic Nitrocellulose for Pseudo-Paper ELISA
Authors: Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
Journal: Analytical Chemistry (2017)
Spinal Cord Inflammation: Molecular Imaging after Thoracic Aortic Ischemia Reperfusion Injury
Authors: Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T
Journal: Radiology (2016): 152222
Myeloperoxidase–Hepatocyte–Stellate Cell Cross Talk Promotes Hepatocyte Injury and Fibrosis in Experimental Nonalcoholic Steatohepatitis
Authors: Pulli, Benjamin and Ali, Muhammad and Iwamoto, Yoshiko and Zeller, Matthias WG and Schob, Stefan and Linnoila, Jenny J and Chen, John W
Journal: Antioxidants & redox signaling (2015): 1255–1269
Myeloperoxidase Nuclear Imaging for Epileptogenesis
Authors: Zhang, Yinian and Seeburg, Daniel P and Pulli, Benjamin and Wojtkiewicz, Gregory R and Bure, Lionel and Atkinson, Wendy and Schob, Stefan and Iwamoto, Yoshiko and Ali, Muhammad and Zhang, Wei and others, undefined
Journal: Radiology (2015): 822–830
Ordered cleavage of myeloperoxidase ester bonds releases active site heme leading to inactivation of myeloperoxidase by benzoic acid hydrazide analogs
Authors: Huang, Jiansheng and Smith, Forrest and Panizzi, Peter
Journal: Archives of biochemistry and biophysics (2014): 74–85
Raising the shields: PCR in the presence of metallic surfaces protected by tailor-made coatings
Authors: Scherag, Frank D and Br, undefined and stetter, Thomas and Rühe, Jürgen
Journal: Colloids and Surfaces B: Biointerfaces (2014): 576–582
Measuring myeloperoxidase activity in biological samples
Authors: Pulli, Benjamin and Ali, Muhammad and Forghani, Reza and Schob, Stefan and Hsieh, Kevin LC and Wojtkiewicz, Gregory and Linnoila, Jenny J and Chen, John W
Journal: PLoS One (2013): e67976
Referencias
Ver todas las 61 referencias: Citation Explorer
Volatile fatty acid-sensing system involving coenzyme-A transferase
Authors: Rajashekhara E, Hosoda A, Sode K, Ikenaga H, Watanabe K.
Journal: Biotechnol Prog (2006): 334
Galactose oxidase action on galactose containing glycolipids–a fluorescence method
Authors: Fortelius M, Mattjus P.
Journal: Chem Phys Lipids (2006): 103
Effects of chronic ethanol administration on the activities and relative synthetic rates of myelin and synaptosomal plasma membrane-associated sialidase in the rat brain
Authors: Azuine MA, Patel SJ, Lakshman MR.
Journal: Neurochem Int (2006): 67
Mitochondrial reactive oxygen species in mice lacking superoxide dismutase 2: attenuation via antioxidant treatment
Authors: Morten KJ, Ackrell BA, Melov S.
Journal: J Biol Chem (2006): 3354
A new, sensitive method for enzyme kinetic studies of scarce glucosides
Authors: Mazura P, Fohlerova R, Brzobohaty B, Kiran NS, J and a L., undefined
Journal: J Biochem Biophys Methods (2006): 55
Evaluation of assays for the measurement of bovine neutrophil reactive oxygen species
Authors: Rinaldi M, Moroni P, Paape MJ, Bannerman DD.
Journal: Vet Immunol Immunopathol. (2006)
Kinetic analysis of semisynthetic peroxidase enzymes containing a covalent DNA-heme adduct as the cofactor
Authors: Fruk L, Muller J, Niemeyer CM.
Journal: Chemistry (2006): 7448
K+-independent actions of diazoxide question the role of inner membrane KATP channels in mitochondrial cytoprotective signaling
Authors: Drose S, Br and t U, Hanley PJ.
Journal: J Biol Chem (2006): 23733
A Role for Mitochondrial Dysfunction in Perpetuating Radiation-Induced Genomic Instability
Authors: Kim GJ, Fiskum GM, Morgan WF.
Journal: Cancer Res (2006): 10377
Sexual Dimorphism in Oxidant Status in Spontaneously Hypertensive Rats
Authors: Sullivan JC, Sasser JM, Pollock JS.
Journal: Am J Physiol Regul Integr Comp Physiol. (2006)
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
FAQ
Can I use azide in ELISA buffers?
What is HRP conjugated secondary antibody?
What label should I choose for a secondary antibody?
Why is an HRP conjugated secondary antibody used in western blotting?
Are any of the cyanine dyes infrared?
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