Rodamina B, éster hexílico, perclorato
Descripción
Los tintes de rodamina cargados positivamente (como los ésteres de rodamina y las rosaminas) se localizan selectivamente en las mitocondrias, por lo que se utilizan ampliamente para marcar mitocondrias de células vivas. Este éster de rodamina en particular tiñe las mitocondrias de naranja con fluorescencia. Sus propiedades espectrales son similares a las de TRITC, lo que hace que el uso de hexil éster de rodamina B sea bastante conveniente.
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-22211 | Rhodamine B, hexyl ester, perchlorate | 10 mg |
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelar (< -15 °C); Minimizar la exposición a la luz.
Espectro
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Propiedades espectrales
| Absorbancia (nm) | 541 |
| Coeficiente de extinción (cm -1 M -1) | 1060001 |
| Exitación | 546 |
| Emisión | 568 |
| Rendimiento cuántico | 0.312 |
Calculadora
Preparación de la solución de stock común
Volumen de DMSO necesario para reconstituir la masa específica de rodamina B, hexil éster, perclorato a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 159.446 µL | 797.232 µL | 1.594 mL | 7.972 mL | 15.945 mL |
| 5 mM | 31.889 µL | 159.446 µL | 318.893 µL | 1.594 mL | 3.189 mL |
| 10 mM | 15.945 µL | 79.723 µL | 159.446 µL | 797.232 µL | 1.594 mL |
Imagen
Figura 1. Estructura química de Rhodamine B, hexyl ester, perchlorate (Rodamina B, éster hexílico, perclorato)
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Bibliografía
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Referencias
Ver todas las 29 referencias: Citation Explorer
Interaction of a mitochondrial membrane potential-sensitive dye, rhodamine 800, with rat mitochondria, cells, and perfused hearts
Authors: Jilkina O, Kong HJ, Hwi L, Kuzio B, Xiang B, Manley D, Jackson M, Kupriyanov VV.
Journal: J Biomed Opt (2006): 14009
Nature and significance of the electron-dense bodies of the endospores of rhinosporidium seeberi: their reactions with MTT (3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide) and TMRE (tetramethyl-rhodamine ethyl ester)
Authors: Arseculeratne SN, Atapattu DN, Wickramaratne K.
Journal: Med Mycol (2005): 261
Photophysical, photochemical, and tumor-selectivity properties of bromine derivatives of rhodamine-123
Authors: Lacerda SH, Abraham B, Stringfellow TC, Indig GL.
Journal: Photochem Photobiol (2005): 1430
Isolation and enrichment of murine spermatogonial stem cells using rhodamine 123 mitochondrial dye
Authors: Lo KC, Brugh VM, 3rd, Parker M, Lamb DJ.
Journal: Biol Reprod (2005): 767
Cadmium-induced mitochondrial membrane-potential dissipation does not necessarily require cytosolic oxidative stress: studies using rhodamine-123 fluorescence unquenching
Authors: Bolduc JS, Denizeau F, Jumarie C.
Journal: Toxicol Sci (2004): 299
Interference of calmidazolium with measurement of mitochondrial membrane potential using the tetraphenylphosphonium electrode or the fluorescent probe rhodamine 123
Authors: Saris NE, Teplova VV, Odinokova IV, Azarashvily TS.
Journal: Anal Biochem (2004): 109
Fluorescence lifetime imaging (FLIM) of rhodamine 123 in living cells
Authors: Schneckenburger H, Stock K, Lyttek M, Strauss WS, Sailer R.
Journal: Photochem Photobiol Sci (2004): 127
Rhodamine 123 as a probe of mitochondrial membrane potential: evaluation of proton flux through F(0) during ATP synthesis
Authors: Baracca A, Sgarbi G, Solaini G, Lenaz G.
Journal: Biochim Biophys Acta (2003): 137
Rhodamine B as a mitochondrial probe for measurement and monitoring of mitochondrial membrane potential in drug-sensitive and -resistant cells
Authors: Reungpatthanaphong P, Dechsupa S, Meesungnoen J, Loetchutinat C, Mankhetkorn S.
Journal: J Biochem Biophys Methods (2003): 1
Microfluorometric study of oxygen dependence of (1″-pyrene butyl)-2-rhodamine ester probe in mitochondria of living cells
Authors: Ribou AC, Vigo J, Kohen E, Salmon JM.
Journal: J Photochem Photobiol B (2003): 107
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