DCFH-DA [diacetato de 2′,7′-diclorodihidrofluoresceína] *CAS 4091-99-0*

Descripción

DCFH-DA, diacetato de 2′,7′-diclorodihidrofluoresceína (también llamado diacetato de 2′,7′-diclorofluorescina), es hidrolizado por esterasas celulares a 2′,7′-diclorodihidrofluoresceína (también llamada 2′,7′-diclorofluorescina) y luego se oxida a 2′,7′-diclorofluoresceína principalmente por H2O2.

El diacetato de 2′,7′-diclorodihidrofluoresceína podría ser reactivo frente a una amplia gama de reacciones oxidantes que pueden aumentar durante el estrés oxidativo intracelular. Esta sonda es ampliamente utilizada para monitorear procesos redox celulares.

Nombre en Ingles: DCFH-DA [2′,7′-Dichlorodihydrofluorescein diacetate] *CAS 4091-99-0*

Catalogo	Producto	Presentación
AAT-15204	DCFH-DA [diacetato de 2′,7′-diclorodihidrofluoresceína]	25 mg

Importante: Producto no diseñado para uso diagnóstico o terapéutico. Solo para uso en investigación (RUO). Almacenamiento a largo plazo: Congelacion a < -15 °C. Minimizar la exposición a la luz.

Plataforma

Microscopio de Fluorescencia

Excitación	Juego de filtros FITC
Emisión	Juego de filtros FITC
Placa Recomendada	Pared negra/fondo claro
Especificaciones instrumento	Juego de filtros FITC

Propiedades Fisicas

Peso Molecular	487.29
Disolvente	DMSO

Espectro

Abrir en Advanced Spectrum Viewer

Propiedades Espectrales

Excitación (nm)	505
Emisión (nm)	526

Calculadora

Preparación de la solución de stock común
Volumen de DMSO necesario para reconstituir la masa específica de DCFH-DA [diacetato de 2′,7′-diclorodihidrofluoresceína] CAS 4091-99-0 a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	205.217 µL	1.026 mL	2.052 mL	10.261 mL	20.522 mL
5 mM	41.043 µL	205.217 µL	410.433 µL	2.052 mL	4.104 mL
10 mM	20.522 µL	102.608 µL	205.217 µL	1.026 mL	2.052 mL

Figura 1. Estructura quimica para DCFH-DA [2′,7′-Dichlorodihydrofluorescein diacetate] *CAS 4091-99-0*

Otros productos

Sonda Dihidrorodamina 123 CAS 109244-58-8

Bibliografias

Ver todas las 15 bibliografias: Citation Explorer

Ketogenic Diet Alleviates Brain Iron Deposition and Cognitive Dysfunction via Nrf2-mediated Ferroptosis pathway in APP/PS1 Mouse
Authors: Qin, Yaya and Bai, Dazhang and Tang, Ming and Zhang, Ming and Zhao, Li and Li, Jia and Yang, Rui and Jiang, Guohui
Journal: Brain Research (2023): 148404

Oral Pyroptosis Nanoinhibitor for the Treatment of Inflammatory Bowel Disease
Authors: Zhu, Zhenxing and Zhou, Dongtao and Yin, Yi and Li, Zhun and Guo, Zhen and Pan, Yongchun and Gao, Yanfeng and Yang, Jingjing and Zhu, Weiming and Song, Yujun and others,
Journal: Nano Research (2023)

Increased oxidative stress contributes to impaired peripheral CD56dimCD57+ NK cells from patients with systemic lupus erythematosus
Authors: Lu, Zhimin and Tian, Yao and Bai, Ziran and Liu, Jiaqing and Zhang, Yan and Qi, Jingjing and Jin, Minli and Zhu, Jie and Li, Xia
Journal: Arthritis research \& therapy (2022): 1–15

Melatonin inhibits NaIO3-induced ARPE-19 cell apoptosis via suppression of HIF-1$\alpha$/BNIP3-LC3B/mitophagy signaling
Authors: Wang, Kai and Chen, Yong-Syuan and Chien, Hsiang-Wen and Chiou, Hui-Ling and Yang, Shun-Fa and Hsieh, Yi-Hsien
Journal: Cell \& bioscience (2022): 1–15

Two-Pronged Intracellular Co-Delivery of Antigen and Adjuvant for Synergistic Cancer Immunotherapy
Authors: Meng, Junli and Zhang, Peisen and Chen, Qizhe and Wang, Zihua and Gu, Yuan and Ma, Jie and Li, Wang and Yang, Chen and Qiao, Yuanyuan and Hou, Yi and others,
Journal: Advanced Materials (2022): 2202168

Protective role of melatonin against testicular damage caused by polymicrobial sepsis in adult rats
Authors: Doganay, Songul and Budak, Ozcan and Toprak, Veysel and Erman, Gulay and Sahin, Arzu
Journal: Ulus Travma Acil Cerrahi Derg (2022): 723–729

Biomimetic nanoparticles directly remodel immunosuppressive microenvironment for boosting glioblastoma immunotherapy
Authors: Wang, Tingting and Zhang, Hao and Qiu, Weibao and Han, Yaobao and Liu, Hanghang and Li, Zhen
Journal: Bioactive Materials (2022)

Phototherapy and anti-GITR antibody-based therapy synergistically reinvigorate immunogenic cell death and reject established cancers
Authors: Sun, Qi and Yang, Zhenzhen and Lin, Meng and Peng, Yiwei and Wang, Rudong and Du, Yitian and Zhou, Yu and Li, Jiajia and Qi, Xianrong
Journal: Biomaterials (2021): 120648

Inhalation of Salvianolic acid B Prevents Fine Particulate Matter-Induced Acute Airway Inflammation and Oxidative Stress by Downregulating the LTR4/MyD88/NLRP3 Pathway
Authors: Li, Liucheng and Kan, Liandi and Xie, Qiangmin
Journal: (2021)

Down–regulating miR-217-5p Protects Cardiomyocytes against Ischemia/Reperfusion Injury by Restoring Mitochondrial Function via Targeting SIRT1
Authors: Qi, Yujuan and Zhang, Kai and Li, Peijun and Wu, Zhenhua
Journal: Inflammation (2020): 1–14

Referencias

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Kinetic analysis of fluorescein and dihydrofluorescein effluxes in tumour cells expressing the multidrug resistance protein, MRP1
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Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2′,7′-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2′,7′-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123
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Experimental research on insulin antibodies. III. Immunohistology of the endocrine pancreas with guinea pig anti-bovine insulin serum, conjugated with fluorescin isothiocyanate.
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Experimental research on insulin antibodies. II. Immunochemical characterization of guinea pig anti-ox insulin serum conjugated with fluorescin isothiocyanate.
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AssayWise

Hydrogen Peroxide Detection
Selecting the right ROS probe
Intracellular Nitric Oxide (NO) Assays
Intracellular Total ROS Activity Assays
Multicolor Intracellular Calcium Detection Probes