La sonda ROS Brite™ DHCF se puede usar convenientemente para monitorear procesos redox celulares para ensayos de multiplexación con anticuerpos marcados con FITC o líneas celulares GFP. Una herramienta valiosa para investigar el estrés oxidativo en diversas patologías.
Descripción
Sonda ROS Brite™ DHCF
Las especies reactivas de oxígeno (ROS) son moléculas químicamente reactivas que contienen oxígeno. Los ejemplos incluyen superóxido, radical hidroxilo, oxígeno singulete y peróxidos. ROS es altamente reactivo debido a la presencia de electrones de capa de valencia no apareados.
Las ROS se forman como un subproducto natural del metabolismo normal del oxígeno y tienen funciones importantes en la señalización celular y la homeostasis. Sin embargo, durante momentos de estrés ambiental (por ejemplo, exposición a los rayos UV o al calor), los niveles de ROS pueden aumentar drásticamente. Esto puede resultar en un daño significativo a las estructuras celulares. Acumulativamente, esto se conoce como estrés oxidativo.
ROS Brite™ DHCF tiene propiedades redox similares a las del diacetato de 2′,7′-diclorodihidrofluoresceína con espectros significativamente desplazados hacia el rojo. ROS Brite™ DHCF es hidrolizado por esterasas celulares para generar la forma reducida no fluorescente que luego se oxida para generar el tinte libre altamente fluorescente principalmente por H2O2. ROS Brite™ DHCF podría ser reactivo frente a una amplia gama de reacciones oxidantes que pueden aumentar durante el estrés oxidativo intracelular.
Esta sonda se puede usar convenientemente para monitorear procesos redox celulares para ensayos de multiplexación con anticuerpos marcados con FITC o líneas celulares GFP. El producto oxidado es altamente fluorescente en las células. ROS Brite™ DHCF proporciona una herramienta valiosa para investigar el estrés oxidativo en diversas patologías.
Nombre en ingles: ROS Brite™ DHCF
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-16053 | Sonda ROS Brite™ DHCF | 1mg |
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelación (< -15 °C). Minimizar la exposición a la luz.
Propiedades fisicas
| Peso Molecular | 701.50 |
| Disolvente | DMSO |
Espectro
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Propiedades Espectrales
| Excitación (nm) | 560 |
| Emisión (nm) | 575 |
Calculadora
Preparación de la solución de stock común
Volumen de DMSO necesario para reconstituir la masa específica de ROS Brite™ DHCF a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 142.552 µL | 712.758 µL | 1.426 mL | 7.128 mL | 14.255 mL |
| 5 mM | 28.51 µL | 142.552 µL | 285.103 µL | 1.426 mL | 2.851 mL |
| 10 mM | 14.255 µL | 71.276 µL | 142.552 µL | 712.758 µL | 1.426 mL |
Imagen
Figura 1. Imágenes de fluorescencia de la medición de ROS en células HeLa utilizando ROS Brite™ DHCF (Cat# 16053). Tratamiento con H2O2: las células se incubaron con ROS Brite™ DHCF durante 1 hora, luego se trataron con H2O2 1 mM a 37 °C durante 30 minutos. Control no tratado: las células HeLa se incubaron con ROS Brite™ DHCF a 37 °C durante 1 hora sin tratamiento con H2O2. La señal de fluorescencia se midió utilizando un microscopio de fluorescencia con un filtro TRITC.
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FAQ
Are NADH and ROS related?
Are there any alternatives for ethidium bromide in agarose gels?
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AssayWise
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