Calcein Red. Calcein AM es una de las sondas fluorescentes más populares que se utilizan para marcar y monitorear las funciones celulares de células vivas.
Descripción
Calcein Red™ sal sódica
Calcein AM es una de las sondas fluorescentes más populares que se utilizan para marcar y monitorear las funciones celulares de células vivas.
Sin embargo, el color único de Calcein AM hace imposible utilizar este valioso reactivo en aplicaciones multicolores. Por ejemplo, es imposible utilizar Calcein AM en combinación de células transfactadas con GFP debido al mismo color que la GFP. Para abordar esta limitación de color de Calcein AM, hemos desarrollado Calcein Orange™, Calcein Red™ y Calcein Red™.
Estos nuevos análogos de Calcein AM permiten el etiquetado multicolor y el análisis funcional de células vivas en combinación con Calcein AM. Calcein Red™ AM no fluorescente puede penetrar fácilmente en las células vivas y se hidroliza para generar el tinte Calcein Red™ fuertemente fluorescente.
El tinte Calcein Red™ se puede controlar con el juego de filtros común TRITC/Cy3. AAT Bioquest también ofrece Calcein Red™ como tinte de referencia para Calcein Red™ AM.
Nombre en Ingles: Calcein Red™ sodium salt
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-21901 | Calcein Red™ sodium salt | 1 mg |
Importante, Solo para uso en investigación (RUO). Almacenamiento a largo plazo: Congelar a < -15 °C. Minimizar la exposición a la luz.
Espectro
Abrir en Advanced Spectrum Viewer
Propiedades Espectrales
| Excitación (nm) | 562 |
| Emisión (nm) | 576 |
Propiedades Fisicas
| Peso Molecular | 825.44 |
| Solvente | Agua |
Calculadora
Preparación de solución madre común
Tabla 1. Volumen de agua necesario para reconstituir la masa específica de sal sódica de Calcein Red™ a una concentración determinada. Tenga en cuenta que el volumen es sólo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los tampones experimentales/fisiológicos adecuados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 121.148 µL | 605.738 µL | 1.211 mL | 6.057 mL | 12.115 mL |
| 5 mM | 24.23 µL | 121.148 µL | 242.295 µL | 1.211 mL | 2.423 mL |
| 10 mM | 12.115 µL | 60.574 µL | 121.148 µL | 605.738 µL | 1.211 mL |
Imagenes
Figura 1: Espectro para Calcein Red
Productos Relacionados
| Name | Excitation (nm) | Emission (nm) |
| Calcein Orange™ sodium salt | 531 | 545 |
Otros Productos
Bibliografia
Ver todas las 17 bibliografias: Citation Explorer
Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice
Authors: Li, Jingcheng and Zhang, Jianxiong and Wang, Meng and Pan, Junxia and Chen, Xiaowei and Liao, Xiang
Journal: Biomedical Optics Express (2017): 2599–2610
NINJ2–A novel regulator of endothelial inflammation and activation
Authors: Wang, Jingjing and Fa, Jingjing and Wang, Pengyun and Jia, Xinzhen and Peng, Huixin and Chen, Jing and Wang, Yifan and Wang, Chenhui and Chen, Qiuyun and Tu, Xin and others, undefined
Journal: Cellular Signalling (2017)
Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B
Authors: Bogert, Nicolai V and Werner, Isabella and Kornberger, Angela and Meybohm, Patrick and Moritz, Anton and Keller, Till and Stock, Ulrich A and Beiras-Fern, undefined and ez, Andres
Journal: Scientific reports (2016)
Inhibition of ABC transport proteins by oil sands process affected water
Authors: Alharbi, Hattan A and Saunders, David MV and Al-Mousa, Ahmed and Alcorn, Jane and Pereira, Alberto S and Martin, Jonathan W and Giesy, John P and Wiseman, Steve B
Journal: Aquatic Toxicology (2016): 81–88
Rapid generation of collagen-based microtissues to study cell–matrix interactions
Authors: Brett, Marie-Elena and Crampton, Alex and ra L , undefined and Wood, David K
Journal: Technology (2016): 1–8
Toxicokinetics and toxicodynamics of chlorpyrifos is altered in embryos of Japanese medaka exposed to oil sands process-affected water: evidence for inhibition of P-glycoprotein
Authors: Alharbi, Hattan A and Alcorn, Jane and Al-Mousa, Ahmed and Giesy, John P and Wiseman, Steve B
Journal: Journal of Applied Toxicology (2016)
Flexible Endoscopic Spray Application of Respiratory Epithelial Cells as Platform Technology to Apply Cells in Tubular Organs
Authors: Thiebes, Anja Lena and Reddemann, Manuel Armin and Palmer, Johannes and Kneer, Reinhold and Jockenhoevel, Stefan and Cornelissen, Christian Gabriel
Journal: Tissue Engineering Part C: Methods (2016): 322–331
Erythropoietin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in an Aneurysm Neck After Coil Embolization by Modulating Vascular Endothelial Growth Factor
Authors: Liu, Peixi and Zhou, Yingjie and An, Qingzhu and Song, Yaying and Chen, Xi and Yang, Guo-Yuan and Zhu, Wei
Journal: MEDICINE (2016): 1–8
Spraying respiratory epithelial cells to coat tissue-engineered constructs
Authors: Thiebes, Anja Lena and Albers, Stefanie and Klopsch, Christian and Jockenhoevel, Stefan and Cornelissen, Christian G
Journal: BioResearch open access (2015): 278–287
Visualization of adherent cell monolayers by cryo-electron microscopy: A snapshot of endothelial adherens junctions
Authors: Le Bihan, Olivier and Decossas, Marion and Gontier, Etienne and Gerbod-Giannone, Marie-Christine and Lambert, Olivier
Journal: Journal of structural biology (2015): 470–477
Referencias
Ver todas las 84 referencias: Citation Explorer
Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development
Authors: Li, Deng and Xiang, Xiaocong and Yang, Fei and Xiao, Dongqin and Liu, Kang and Chen, Zhu and Zhang, Ruolan and Feng, Gang
Journal: Biochemical and Biophysical Research Communications (2017)
Localized functional chemical stimulation of TE 671 cells cultured on nanoporous membrane by calcein and acetylcholine
Authors: Zibek S, Stett A, Koltay P, Hu M, Zengerle R, Nisch W, Stelzle M.
Journal: Biophys J. (2006)
A vaccination and challenge model using calcein marked fish
Authors: Klesius PH, Evans JJ, Shoemaker CA, Pasnik DJ.
Journal: Fish Shellfish Immunol (2006): 20
Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging
Authors: Bratosin D, Mitrofan L, Palii C, Estaquier J, Montreuil J.
Journal: Cytometry A (2005): 78
Cytotoxic effects of 100 reference compounds on Hep G2 and HeLa cells and of 60 compounds on ECC-1 and CHO cells. I mechanistic assays on ROS, glutathione depletion and calcein uptake
Authors: Schoonen WG, Westerink WM, de Roos JA, Debiton E.
Journal: Toxicol In Vitro (2005): 505
Calcein AM release-based cytotoxic cell assay for fish leucocytes
Authors: Iwanowicz LR, Densmore CL, Ottinger CA.
Journal: Fish Shellfish Immunol (2004): 127
Calcein-AM is a detector of intracellular oxidative activity
Authors: Uggeri J, Gatti R, Belletti S, Sc and roglio R, Corradini R, Rotoli BM, Orl and ini G., undefined
Journal: Histochem Cell Biol (2004): 499
Comparison of the usefulness of the MTT, ATP, and calcein assays to predict the potency of cytotoxic agents in various human cancer cell lines
Authors: Mueller H, Kassack MU, Wiese M.
Journal: J Biomol Screen (2004): 506
In vitro assay of mineralized-tissue formation on titanium using fluorescent staining with calcein blue
Authors: Goto T, Kajiwara H, Yoshinari M, Fukuhara E, Kobayashi S, Tanaka T.
Journal: Biomaterials (2003): 3885
The effects of calcium chloride and sodium chloride on the electroporation-mediated skin permeation of fluorescein isothiocyanate (FITC)-dextrans in vitro
Authors: Tokudome Y, Sugibayashi K.
Journal: Biol Pharm Bull (2003): 1508
Application notes
Experimental Protocol for Calcein AM Assay
Annexin V
Calcein
Calcein AM Assay Standardization
Multi-Color Labeling and Functional Analysis of Live Cells Using Fluorescent Calcein AM Dyes
FAQ
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there upgraded trypan blue derivatives for cell viability testing?
Is Calcein, AM toxic to cells?
What are common laser lines used in flow cytometry?
What does trypan blue do?
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