Kit de ensayo Cell Meter™ de apoptosis de fosfatidilserina (Fluorescencia verde) está diseñado para monitorear la apoptosis celular midiendo la translocación de fosfatidilserina (PS).
Descripción
Kit de ensayo Cell Meter™ de apoptosis de fosfatidilserina, Fluorescencia verde optimizada para lectores de microplacas
Los kits de ensayo Cell Meter™ son un conjunto de herramientas para monitorear la viabilidad celular. Existe una variedad de parámetros que se pueden utilizar para monitorear la viabilidad celular.
Este kit en particular está diseñado para monitorear la apoptosis celular midiendo la translocación de fosfatidilserina (PS). En la apoptosis, la PS se transfiere a la valva externa de la membrana plasmática. La aparición de fosfatidilserina en la superficie celular es un indicador universal de las etapas inicial/intermedia de la apoptosis celular y puede detectarse antes de que se puedan observar cambios morfológicos.
El kit está diseñado para monitorear la apoptosis celular midiendo la translocación de fosfatidilserina (PS), y utiliza nuestro sensor de PS Apopxin™ basado en moléculas pequeñas fluorescentes patentado que se une específicamente a PS con una afinidad mucho mayor que la anexina V (Kd < 10 nM). Tiene fluorescencia verde al unirse a la membrana PS. Se puede utilizar en formatos de microplaca, microscopio y citómetro de flujo, mientras que la mayoría de los demás kits de ensayo de apoptosis comerciales solo se utilizan con microscopio o plataforma de citometría de flujo.
Nombre en Ingles: Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Green Fluorescence Optimized for Microplate Readers*
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-22791 | Kit de ensayo Cell Meter™ de apoptosis de fosfatidilserina | 100 pruebas |
Componentes
| Component A: Apopxin™ Green (100X Stock Solution) | 1 vial (100 µL/vial) |
| Component B: Assay Buffer (4 °C) | 1 frasco (10 mL) |
Importante, Solo para uso en investigación (RUO). Almacenamiento: Refrigeración de 2-8 °C. Minimizar la exposición a la luz.
Plataforma
Lector de fluorescencia de microplacas
| Excitación | 490 nm |
| Emisión | 525 nm |
| Cutoff | 515 nm |
| Placa recomendada | Pared negra, fondo claro |
| Especificaciones Instrumento | Modo lectura inferior |
Imagenes
Figura 1. Detección de actividad de unión a fosfatidilserina en células Jurkat. Se sembraron células Jurkat el mismo día a razón de 200.000 células/90 µl/pocillo en una placa de 96 pocillos de pared negra/fondo transparente Costar. Las células se trataron con diferentes dosis de camptotecina durante 5 horas como se indica. Se añadió la solución de ensayo Apopxin™ Green (100 µl/pocillo) y se incubó a temperatura ambiente durante 1 hora. La intensidad de la fluorescencia se midió a Ex/Em = 490/525 nm con el instrumento NOVOstar (de BMG Labtech) usando el modo de lectura inferior.
Figura 1: Imágenes de células Jurkat teñidas con el kit de ensayo de apoptosis de fosfatidilserina Cell Meter™ en una placa de 96 pocillos de pared negra/fondo transparente Costar. A: Células de control no tratadas. B: Células tratadas con camptotecina 20 µM durante 5 horas.
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Referencias
Ver todas las 92 referencias: Citation Explorer
Suicidal membrane repair regulates phosphatidylserine externalization during apoptosis
Authors: Mirnikjoo B, Balasubramanian K, Schroit AJ.
Journal: J Biol Chem (2009): 22512
Peptidic targeting of phosphatidylserine for the MRI detection of apoptosis in atherosclerotic plaques
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Detection of apoptosis based on the interaction between annexin V and phosphatidylserine
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Journal: Anal Chem (2009): 2410
Evaluation of cell surface expression of phosphatidylserine in ovarian carcinoma effusions using the annexin-V/7-AAD assay: clinical relevance and comparison with other apoptosis parameters
Authors: Dong HP, Holth A, Kleinberg L, Ruud MG, Elstr and MB, Trope CG, Davidson B, Risberg B.
Journal: Am J Clin Pathol (2009): 756
Mobilization of lysosomal calcium regulates the externalization of phosphatidylserine during apoptosis
Authors: Mirnikjoo B, Balasubramanian K, Schroit AJ.
Journal: J Biol Chem (2009): 6918
Trivalent methylated arsenical-induced phosphatidylserine exposure and apoptosis in platelets may lead to increased thrombus formation
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Journal: Toxicol Appl Pharmacol (2009): 144
Discovery of a phosphatidylserine-recognizing peptide and its utility in molecular imaging of tumour apoptosis
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Journal: J Cell Mol Med (2008): 1649
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Journal: Toxicology (2007): 79
Regulated externalization of phosphatidylserine at the cell surface: implications for apoptosis
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Journal: J Biol Chem (2007): 18357
PET imaging of apoptosis with (64)Cu-labeled streptavidin following pretargeting of phosphatidylserine with biotinylated annexin-V
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Application notes
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V
Calcein
Experimental Protocol for Calcein AM Assay
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FAQ
Are inflammasomes and caspase-1 related?
Do you offer any fluorimetric assays that measure caspase activation/activity in live cells using a flow cytometer?
Does pH and staining temperature affect Annexin V-Phosphatidylserine binding?
Does propidium iodide stain apoptotic cells?
How can I tell if my cell sample is dying?
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