Descripción
Hay varias formas de marcar un oligonucleótido con fluoresceína. La elección de la etiqueta se diversifica aún más, dependiendo de los requisitos espectrales. La fosforamidita de 5′-fluoresceína-CE, derivada del isómero único 6-carboxifluoresceína, la fosforamidita de 5′-hexaclorofluoresceína-CE (HEX) y la fosforamidita de 5′-tetraclorofluoresceína-CE (TET) se pueden usar para marcar eficientemente un oligonucleótido en el 5 ‘-fin. El etiquetado del extremo 3′ de un oligo con fluoresceína se puede lograr utilizando CPG de 3’-fluoresceína. La escisión estándar y la desprotección con hidróxido de amonio liberan el oligo marcado con fluoresceína cuando se usa cualquiera de estos soportes.
Catalogo | Producto | Presentación |
---|---|---|
AAT-6026 | 6-HEX phosphoramidite [5′-Hexachlorofluorescein phosphoramidite] | 100 µmoles |
AAT-6024 | 6-HEX phosphoramidite [5′-Hexachlorofluorescein phosphoramidite] | 10×100 µmoles |
Importante, Solo para uso en investigación (RUO). Almacenamiento: Congelar (< -15 °C), Minimizar la exposición a la luz
Propiedades fisicas
Peso Molecular | 1050.61 |
Disolvente | MeCN |
Calculadora
Preparación de la solución de stock común.
Volumen de MeCN necesario para reconstituir la masa específica de fosforamidita de 6-HEX [fosforamidita de 5′-hexaclorofluoresceína] a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 95.183 µL | 475.914 µL | 951.828 µL | 4.759 mL | 9.518 mL |
5 mM | 19.037 µL | 95.183 µL | 190.366 µL | 951.828 µL | 1.904 mL |
10 mM | 9.518 µL | 47.591 µL | 95.183 µL | 475.914 µL | 951.828 µL |
Espectro
Abrir en Advanced Spectrum Viewer
![](http://cidsamexico.com/wp-content/uploads/2023/01/image-145.png)
Propiedades Espectrales
Factor de corrección (280 nm) | 0.15 |
Coeficiente de extinción (cm -1 M -1) | 73000 |
Excitación (nm) | 533 |
Emisión (nm) | 550 |
Imagenes
![](http://cidsamexico.com/wp-content/uploads/2023/01/image-146-1024x418.png)
Figura 1. Estructura quimica de 6-HEX phosphoramidite [5′-Hexachlorofluorescein phosphoramidite]
Productos Relacionados
Nombre | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
6-HEX, SE [6-Carboxy-2′,4,4′,5′,7,7′-hexachlorofluorescein, succinimidyl ester] *Single Isomer* | 533 | 559 | 73000 | 0.15 |
6-HEX azide | 533 | 559 | 73000 | 0.15 |
6-HEX alkyne | 533 | 559 | 73000 | 0.15 |
6-FAM phosphoramidite [5′-Fluorescein phosphoramidite] *CAS 204697-37-0* | 493 | 517 | 83000 | 0.178 |
6-Fluorescein phosphoramidite | 498 | 517 | 800001 | 0.275 |
6-TET phosphoramidite [5′-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6* | 521 | 542 | 73000 | 0.13 |
6-JOE Phosphoramidite | 520 | 545 | 750002 | – |
HEX-dT Phosphoramidite | – | – | – | – |
Referencias
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Application Notes (en Ingles)
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
Calcein