Proteina AChR T. Californica

Descripción

ProteinsOur AChR se purifica directamente del receptor de acetilcolina de Torpedo californica. Es el mejor ejemplo de cómo se pueden usar perlas de purificación de proteínas personalizadas para aislar una proteína sin ninguna etiqueta de epítopo. La proteína se purificó mediante resina de agarosa acoplada a neurotoxina-3 de cobra real, un ligando natural de AChR.

Catalogo	Producto	Presentación
CU-28601	Proteina AChR T. Californica	40 ug
CU-28603	Proteina AChR T. Californica	80 ug

Caracteristicas

Alternative names	Acetylcholine receptor subunit alpha, beta, gamma and delta
UniProt Number	P02712 (alpha subunit)P02714 (beta subunit)P02718 (gamma subunit)P02710 (delta subunit)
Protein class	Ion channel, Ligand-gated ion channel, Receptor
Organism	Torpedo californica (electric ray)
Sequence, Alpha subunit One-Letter Code	MILCSYWHV GLVLLLFSCC GLVLGSEHET RLVANLLENY NKVIRPVEHH THFVDITVGL QLIQLISVDE VNQIVETNVR LRQQWIDVRL RWNPADYGGI KKIRLPSDDV WLPDLVLYNN ADGDFAIVHM TKLLLDYTGK IMWTPPAIFK SYCEIIVTHF PFDQQNCTMK LGIWTYDGTK VSISPESDRP DLSTFMESGE WVMKDYRGWK HWVYYTCCPD TPYLDITYHF IMQRIPLYFV VNVIIPCLLF SFLTGLVFYL PTDSGEKMTL SISVLLSLTV FLLVIVELIP STSSAVPLIG KYMLFTMIFV ISSIIITVVV INTHHRSPST HTMPQWVRKI FIDTIPNVMF FSTMKRASKE KQENKIFADD IDISDISGKQ VTGEVIFQTP LIKNPDVKSA IEGVKYIAEH MKSDEESSNA AEEWKYVAMV IDHILLCVFM LICIIGTVSV FAGRLIELSQ EG
Sequence, Beta subunit One-Letter Code	MENVRRMALG LVVMMALALS GVGASVMEDT LLSVLFETYN PKVRPAQTVG DKVTVRVGLT LTNLLILNEK IEEMTTNVFL NLAWTDYRLQ WDPAAYEGIK DLRIPSSDVW QPDIVLMNNN DGSFEITLHV NVLVQHTGAV SWQPSAIYRS SCTIKVMYFP FDWQNCTMVF KSYTYDTSEV TLQHALDAKG EREVKEIVIN KDAFTENGQW SIEHKPSRKN WRSDDPSYED VTFYLIIQRK PLFYIVYTII PCILISILAI LVFYLPPDAG EKMSLSISAL LAVTVFLLLL ADKVPETSLS VPIIIRYLMF IMILVAFSVI LSVVVLNLHH RSPNTHTMPN WIRQIFIETL PPFLWIQRPV TTPSPDSKPT IISRANDEYF IRKPAGDFVC PVDNARVAVQ PERLFSEMKW HLNGLTQPVT LPQDLKEAVE AIKYIAEQLE SASEFDDLKK DWQYVAMVAD RLFLYVFFVI CSIGTFSIFL DASHNVPPDN PFA
Sequence, Gamma subunit One-Letter Code	MVLTLLLIIC LALEVRSENE EGRLIEKLLG DYDKRIIPAK TLDHIIDVTL KLTLTNLISL NEKEEALTTN VWIEIQWNDY RLSWNTSEYE GIDLVRIPSE LLWLPDVVLE NNVDGQFEVA YYANVLVYND GSMYWLPPAI YRSTCPIAVT YFPFDWQNCS LVFRSQTYNA HEVNLQLSAE EGEAVEWIHI DPEDFTENGE WTIRHRPAKK NYNWQLTKDD TDFQEIIFFL IIQRKPLFYI INIIAPCVLI SSLVVLVYFL PAQAGGQKCT LSISVLLAQT IFLFLIAQKV PETSLNVPLI GKYLIFVMFV SMLIVMNCVI VLNVSLRTPN THSLSEKIKH LFLGFLPKYL GMQLEPSEET PEKPQPRRRS SFGIMIKAEE YILKKPRSEL MFEEQKDRHG LKRVNKMTSD IDIGTTVDLY KDLANFAPEI KSCVEACNFI AKSTKEQNDS GSENENWVLI GKVIDKACFW IALLLFSIGT LAIFLTGHFN QVPEFPFPGD PRKYVP
Sequence, Delta subunit One-Letter Code	MGNIHFVYLLISCLYYSGCS GVNEEERLIN DLLIVNKYNK HVRPVKHNNE VVNIALSLTL SNLISLKETD ETLTSNVWMD HAWYDHRLTW NASEYSDISI LRLPPELVWI PDIVLQNNND GQYHVAYFCN VLVRPNGYVT WLPPAIFRSS CPINVLYFPF DWQNCSLKFT ALNYDANEIT MDLMTDTIDG KDYPIEWIII DPEAFTENGE WEIIHKPAKK NIYPDKFPNG TNYQDVTFYL IIRRKPLFYV INFITPCVLI SFLASLAFYL PAESGEKMST AISVLLAQAV FLLLTSQRLP ETALAVPLIG KYLMFIMSLV TGVIVNCGIV LNFHFRTPST HVLSTRVKQI FLEKLPRILH MSRADESEQP DWQNDLKLRR SSSVGYISKA QEYFNIKSRS ELMFEKQSER HGLVPRVTPR IGFGNNNENI AASDQLHDEI KSGIDSTNYI VKQIKEKNAY DEEVGNWNLV GQTIDRLSMF IITPVMVLGT IFIFVMGNFN HPPAKPFEGD PFDYSSDHPR CA
Affinity tags	none
Expression Host	T. californica (native)
Size (excluding additional elements)	65 kDA
Buffer composition	137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 5 % glycerin; 0,1 % Triton
Purity	>95%, determined via SDS-PAGE
Purified via	Protein-specific affinity matrix using immobilized agonist ligand followed by dialysis
Function	After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Preparación
Expression system: T. Californica (native)
Purification: Protein-specific affinity matrix using immobilized agonist ligand, dialysis
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 5 % glycerin;
0,1 % Triton
Activity: Native AChR from T.Californica, purified as funtional heterotetramer binding to
Neurotoxin-3 ligand
Form: Liquid

Control de Calidad
Purity: (SDS-PAGE) >95% as determined by SDS-PAGE, see Fig. 1
Activity: Purified as funtional heterotetramer binding to Neurotoxin-3 ligand

Aplicaciones
• SDS-PAGE
• Western Blot
• Ligand binding assays (e.g. SPR)
• Protein Crystallization
• Biochemical and biophysical analyses
• Immunization

Envio y Almacenamiento

Este producto se envia en hielo seco y se almacena a -80°C. Evitar ciclos de congelamiento y descongelamiento.

Imagenes

Descargo de responsabilidad: Nuestros productos están destinados para investigacion en aplicaciones de biología molecular. Estos productos no están destinados al diagnóstico, prevención o tratamiento de una enfermedad.