6-FAM phosphoramidite [5′-Fluorescein phosphoramidite]

Fosforamidita de 6-FAM [fosforamidita de 5′-fluoresceína] *CAS 204697-37-0*

Descripción

Hay varias formas de marcar un oligonucleótido con fluoresceína. La elección de la etiqueta se diversifica aún más, dependiendo de los requisitos espectrales. La fosforamidita de 5′-fluoresceína-CE, derivada del isómero único 6-carboxifluoresceína, la fosforamidita de 5′-hexaclorofluoresceína-CE (HEX) y la fosforamidita de 5′-tetraclorofluoresceína-CE (TET) se pueden usar para marcar eficientemente un oligonucleótido en el 5 ‘-fin. El etiquetado del extremo 3′ de un oligo con fluoresceína se puede lograr utilizando CPG de 3’-fluoresceína. La escisión estándar y la desprotección con hidróxido de amonio liberan el oligo marcado con fluoresceína cuando se usa cualquiera de estos soportes.

Catalogo	Producto	Presentación
AAT-6016	6-FAM phosphoramidite [5′-Fluorescein phosphoramidite]	100 µmoles
AAT-6017	6-FAM phosphoramidite [5′-Fluorescein phosphoramidite]	10×100 µmoles

SDS

Protocol

Importante, Solo para uso en investigación (RUO). Almacenamiento: Congelar (< -15 °C), Minimizar la exposición a la luz

Propiedades fisicas

Peso Molecular	843.94
Disolvente	MeCN

Calculadora

Preparación de la solución de stock común.
Volumen de MeCN necesario para reconstituir la masa específica de fosforamidita de 6-FAM [fosforamidita de 5′-fluoresceína] CAS 204697-37-0 a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	118.492 µL	592.459 µL	1.185 mL	5.925 mL	11.849 mL
5 mM	23.698 µL	118.492 µL	236.984 µL	1.185 mL	2.37 mL
10 mM	11.849 µL	59.246 µL	118.492 µL	592.459 µL	1.185 mL

Espectro

Abrir en Advanced Spectrum Viewer

Propiedades Espectrales

Factor de corrección (260 nm)	0.32
Factor de corrección (280 nm)	0.178
Coeficiente de extinción (cm ^-1 M ^-1)	83000
Excitación (nm)	493
Emisión (nm)	517

Imagen

Figura 1. Estructura quimica de 6-FAM phosphoramidite [5′-Fluorescein phosphoramidite] *CAS 204697-37-0*

Productos Relacionados

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
5(6)-FAM [5-(and-6)-Carboxyfluorescein] CAS 72088-94-9	493	517	83000	0.32	0.178
6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] CAS 92557-81-8	493	517	83000	0.32	0.178
6-FAM Azide	493	517	83000	0.32	0.178
6-FAM Alkyne	493	517	83000	0.32	0.178
6-Fluorescein phosphoramidite	498	517	80000¹	0.32	0.275
6-TET phosphoramidite [5′-Tetrachlorofluorescein phosphoramidite] CAS#: 877049-90-6	521	542	73000	–	0.13
6-HEX phosphoramidite [5′-Hexachlorofluorescein phosphoramidite]	533	559	73000	–	0.15
6-JOE Phosphoramidite	520	545	75000¹	–	–
FAM-xtra™ Phosphoramidite	493	517	83000	0.32	0.178

Otros Productos Relacionados

5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5*

6-FAM [6-Carboxyfluorescein] *CAS 3301-79-9*

5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, succinimidyl ester] *CAS 117548-22-8*

5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] *CAS 92557-80-7*

5-SFX [5-FAM-X, SE] *CAS 148356-00-7*

5(6)-FAM ethylenediamine

5-FAM ethylenediamine

FMOC-Lys(5-FAM)-OH *CAS 1242933-88-5*

5-FAM-YVAD-FMK

Fluorescein aldehyde [5-FAM aldehyde]

Referencias

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Application Notes (en Ingles)

A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
Calcein