6-FAM phosphoramidite [5′-Fluorescein phosphoramidite]

Fosforamidita de 6-FAM [fosforamidita de 5′-fluoresceína] *CAS 204697-37-0*

Descripción

Hay varias formas de marcar un oligonucleótido con fluoresceína. La elección de la etiqueta se diversifica aún más, dependiendo de los requisitos espectrales. La fosforamidita de 5′-fluoresceína-CE, derivada del isómero único 6-carboxifluoresceína, la fosforamidita de 5′-hexaclorofluoresceína-CE (HEX) y la fosforamidita de 5′-tetraclorofluoresceína-CE (TET) se pueden usar para marcar eficientemente un oligonucleótido en el 5 ‘-fin. El etiquetado del extremo 3′ de un oligo con fluoresceína se puede lograr utilizando CPG de 3’-fluoresceína. La escisión estándar y la desprotección con hidróxido de amonio liberan el oligo marcado con fluoresceína cuando se usa cualquiera de estos soportes.

CatalogoProductoPresentación
AAT-60166-FAM phosphoramidite [5′-Fluorescein phosphoramidite]100 µmoles
AAT-60176-FAM phosphoramidite [5′-Fluorescein phosphoramidite]10×100 µmoles

Importante, Solo para uso en investigación (RUO). Almacenamiento: Congelar  (< -15 °C), Minimizar la exposición a la luz

Propiedades fisicas

Peso Molecular 843.94
DisolventeMeCN

Calculadora

Preparación de la solución de stock común.
Volumen de MeCN necesario para reconstituir la masa específica de fosforamidita de 6-FAM [fosforamidita de 5′-fluoresceína] CAS 204697-37-0 a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM118.492 µL592.459 µL1.185 mL5.925 mL11.849 mL
5 mM23.698 µL118.492 µL236.984 µL1.185 mL2.37 mL
10 mM11.849 µL59.246 µL118.492 µL592.459 µL1.185 mL

Espectro

Abrir en Advanced Spectrum Viewer

Propiedades Espectrales

Factor de corrección (260 nm)0.32
Factor de corrección (280 nm)0.178
Coeficiente de extinción (cm -1 M -1)83000
Excitación (nm)493
Emisión (nm)517

Imagen

Figura 1. Estructura quimica de 6-FAM phosphoramidite [5′-Fluorescein phosphoramidite] *CAS 204697-37-0*

Productos Relacionados

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Referencias

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Application Notes (en Ingles)

A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
Calcein