Equivalente a Cy3® NHS ester
Descripción
Se ha utilizado una variedad de tintes de cianina 3 (Cy3®) para marcar moléculas biológicas para imágenes de fluorescencia y otros análisis bioquímicos basados en fluorescencia. Se utilizan ampliamente para marcar péptidos, proteínas y oligos, etc. Los tintes Cy3® tienen una fluorescencia mejorada al unirse a las proteínas. El éster Cy3® NHS reacciona fácilmente con los grupos amino.
AAT Bioquest ofrece ésteres de colorante Cy NHS en forma de sales de trietilamonio que son más solubles en DMSO y DMF que las sales de potasio correspondientes que ofrecen otros proveedores. Las sales de trietilamonio del tinte Cy tienen la misma reactividad y dan los conjugados idénticos a las sales de potasio del tinte Cy. Cy3® es la marca registrada de GE Healthcare.
| Catálogo | Producto | Presentación |
|---|---|---|
| AAT-141 | Cyanine 3 monosuccinimidyl ester | 1mg |
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelacion (< -15 °C). Minimizar la exposición a la luz.
Espectro
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Propiedades fisicas
| Peso Molecular | 829.03 |
| Disolvente | DMSO |
Propiedades Espectrales
| Factor de corrección (260 nm) | 0.07 |
| Factor de corrección (280 nm) | 0.073 |
| Coeficiente extinción (cm -1 M -1) | 1500001 |
| Excitación (nm) | 555 |
| Emisión (nm) | 569 |
| Rendimiento Cuántico | 0.151 |
Calculadora
Preparación de la solución de stock común
Volumen de DMSO necesario para reconstituir la masa específica del éster monosuccinimidíli de cianina 3 [equivalente al éster Cy3® NHS] a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 120.623 µL | 603.114 µL | 1.206 mL | 6.031 mL | 12.062 mL |
| 5 mM | 24.125 µL | 120.623 µL | 241.246 µL | 1.206 mL | 2.412 mL |
| 10 mM | 12.062 µL | 60.311 µL | 120.623 µL | 603.114 µL | 1.206 mL |
Figura 1. Las células HeLa se incubaron con (Tubulina+) o sin (Tubulina-) anti-tubulina de ratón seguido de conjugado de IgG anti-ratón de cabra Cy3® de AAT (Rojo, Izquierda) o IgG anti-ratón de cabra de Jackson conjugado con Cy3® (Rojo , Derecha), respectivamente. Los núcleos celulares se tiñeron con Hoechst 33342 (Blue, Cat# 17530).
Figura 2. Estructura química del éster monosuccinimidílico de cianina 3 [equivalente al éster Cy3® NHS]
Productos Relacionados
| Nombre | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (482 nm) | Correction Factor (565 nm) |
| Cyanine 3 bissuccinimidyl ester [equivalent to Cy3® bisNHS ester] | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 | – | – |
| Cyanine 5 monosuccinimidyl ester [equivalent to Cy5® NHS ester] | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
| Cyanine 7 monosuccinimidyl ester [equivalent to Cy7® NHS ester] | 756 | 779 | 250000 | 0.3 | 0.05 | 0.036 | 0.0005 | 0.0193 |
Bibliografía
Ver todas las 28 bibliografías: Citation Explorer
A liquid-to-solid phase transition of Cu/Zn superoxide dismutase 1 (SOD1) initiated by oxidation and disease mutation
Authors: Gu, Siyu and Xu, Ming and Chen, Long and Shi, Xiangyan and Luo, Shi-Zhong
Journal: Journal of Biological Chemistry (2022): 102857
Arc weakens synapses by dispersing AMPA receptors from postsynaptic density via modulating PSD phase separation
Authors: Chen, Xudong and Jia, Bowen and Araki, Yoichi and Liu, Bian and Ye, Fei and Huganir, Richard and Zhang, Mingjie
Journal: Cell Research (2022): 914–930
Caveat fluorophore: an insiders’ guide to small-molecule fluorescent labels
Authors: Grimm, Jonathan B and Lavis, Luke D
Journal: Nature methods (2022): 149–158
Phase separation-mediated condensation of Whirlin-Myo15-Eps8 stereocilia tip complex
Authors: Lin, Lin and Shi, Yingdong and Wang, Mengli and Wang, Chao and Lu, Qing and Zhu, Jinwei and Zhang, Rongguang
Journal: Cell Reports (2021): 108770
A simple lectin-based biochip might display the potential clinical value of glycomics in patients with spontaneous intracerebral hemorrhage
Authors: Ye, Lei and Fang, Yong-Sheng and Li, Xiao-Xue and Gao, Yi and Liu, Sheng-Sheng and Chen, Qiang and Wu, Qiang and Cheng, Hong-Wei and Du, Wei-Dong
Journal: Annals of Translational Medicine (2021)
Kindlin2-mediated phase separation underlies integrin adhesion formation
Authors: Li, Yujie and Zhang, Ting and Li, Huadong and Yang, Haibin and Lin, Ruihong and Sun, Kang and Wang, Lei and Zhang, Jing and Wei, Zhiyi and Yu, Cong
Journal: BioRxiv (2020)
Establishment of a 1, 4, 7, 10-tetraazacyclododecane-1, 4, 7, 10-tetraacetic acid mono-N-hydroxysuccinimide ester (DOTA-NHS-ester) based lectin microarray for efficiently detecting serum glycans in gastric cancers
Authors: Gao, Yi and Li, Song-Guo and Liu, Qian and Liu, Sheng-Sheng and Ye, Lei and Song, Zi-Jian and Du, Wei-Dong
Journal: Analytical Biochemistry (2020): 113686
Par complex cluster formation mediated by phase separation
Authors: Liu, Ziheng and Yang, Ying and Gu, Aihong and Xu, Jiawen and Mao, Ying and Lu, Haojie and Hu, Weiguo and Lei, Qun-Ying and Li, Zhouhua and Zhang, Mingjie and others,
Journal: Nature communications (2020): 1–18
Phase separation-mediated formation of condensed GIT/PIX enzyme complex module for compartmentalized signaling
Authors: Zhu, Jinwei and Zhou, Qingqing and Xia, Yitian and Lin, Lin and Li, Jianchao and Peng, Mengjuan and Zhang, Rongguang and Zhang, Mingjie
Journal: bioRxiv (2019)
Role of flagellar hydrogen bonding in Salmonella motility and flagellar polymorphic transition
Authors: Wang, Chu and Lunelli, Michele and Zschieschang, Erik and Bosse, Jens and Thuenauer, Rol and , undefined and Kolbe, Michael
Journal: Molecular microbiology (2019)
Referencias
Ve todas las 21 referencias: Citation Explorer
Excitation of Cy5 in self-assembled lipid bilayers using optical microresonators
Authors: Freeman LM, Li S, Dayani Y, Choi HS, Malmstadt N, Armani AM.
Journal: Appl Phys Lett (2011): 143703
Theranostic cRGD-BioShuttle Constructs Containing Temozolomide- and Cy7 For NIR-Imaging and Therapy
Authors: Wiessler M, Hennrich U, Pipkorn R, Waldeck W, Cao L, Peter J, Ehemann V, Semmler W, Lammers T, Braun K.
Journal: Theranostics (2011): 381
Rational approach to select small peptide molecular probes labeled with fluorescent cyanine dyes for in vivo optical imaging
Authors: Berezin MY, Guo K, Akers W, Livingston J, Solomon M, Lee H, Liang K, Agee A, Achilefu S.
Journal: Biochemistry (2011): 2691
In vivo detection of embryonic stem cell-derived cardiovascular progenitor cells using Cy3-labeled Gadofluorine M in murine myocardium
Authors: Adler ED, Bystrup A, Briley-Saebo KC, Mani V, Young W, Giovanonne S, Altman P, Kattman SJ, Frank JA, Weinmann HJ, Keller GM, Fayad ZA.
Journal: JACC Cardiovasc Imaging (2009): 1114
Quantitative proteomics by fluorescent labeling of cysteine residues using a set of two cyanine-based or three rhodamine-based dyes
Authors: Volke D, Hoffmann R.
Journal: Electrophoresis (2008): 4516
Surface plasmon resonance-enhanced fluorescence implementation of a single-step competition assay: demonstration of fatty acid measurement using an anti-fatty acid monoclonal antibody and a Cy5-labeled fatty acid
Authors: Vareiro MM, Tranchant I, Maplin S, Zak K, Gani MM, Slevin CJ, Hailes HC, Tabor AB, Cameron PJ, Jenkins AT, Williams DE.
Journal: Anal Biochem (2008): 243
Thiazole orange and Cy3: improvement of fluorescent DNA probes with use of short range electron transfer
Authors: Menacher F, Rubner M, Berndl S, Wagenknecht HA.
Journal: J Org Chem (2008): 4263
Near-infrared fluorescence imaging of tumor integrin alpha v beta 3 expression with Cy7-labeled RGD multimers
Authors: Wu Y, Cai W, Chen X.
Journal: Mol Imaging Biol (2006): 226
Cy7-Bis-dipicolylamine-zinc
Authors: Leung K., undefined
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)
Cy7-Tetrameric arginine-glycine-aspartic acid peptide
Authors: Cheng KT., undefined
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)
Application Notes (en Ingles)
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)