Este kit PhosphoWorks™ es de Resplandor continuo y proporciona un ensayo de luminiscencia rápido, simple y homogéneo para la determinación de la proliferación celular y la citotoxicidad en células de mamíferos.
Descripción
El trifosfato de adenosina (ATP) juega un papel fundamental en la energenia celular, la regulación metabólica y la señalización celular. El ensayo de ATP luminometrico PhosphoWorks™ proporciona un kit de luminiscencia rápido, simple y homogéneo para la determinación de la proliferación celular y la citotoxicidad en células de mamíferos.
El ensayo se puede realizar en un conveniente formato de placa de microtitulación de 96 pocillos y 384 pocillos. La alta sensibilidad de este ensayo permite la detección de ATP en muchos sistemas biológicos, muestras ambientales y alimentos. Este kit PhosphoWorks ATP tiene una señal de luminiscencia estable de hasta 4 horas. Tiene una luminiscencia estable sin necesidad de mezclas ni separaciones, y está formulado para tener un tiempo mínimo de manipulación.
Nombre en Ingles: PhosphoWorks™ Luminometric ATP Assay Kit *Extended Luminescence*
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-21608 | Ensayo de ATP luminométrico PhosphoWorks™ | 10 placas |
| AAT-21609 | Ensayo de ATP luminométrico PhosphoWorks™ | 1 placa |
Importante: Solo para uso en investigación (RUO).
Plataforma
Lector de Microplacas de Fluorescencia
| Placa recomendada | Blanca sólida |
Componentes
| Componente A: ATP Monitoring Enzyme | 1 vial |
| Componente B: ATP Sensor (Light-sensitive) | 10 viales |
| Componente C: Buffer de reacción | 2 viales (50 mL/vial) |
PREPARACION DE SOLUCION DE TRABAJO
- Transfiera todo el vial de 10 ml de buffer de reacción (componente C) al sensor de ATP (componente B) y mezcle bien.
- Agregue 20 µL de Enzima de Monitoreo de ATP (Componente A) en la botella de Componente B+C y mezcle bien para hacer una solución de trabajo de ATP. Nota: Evite la posible contaminación por ATP de fuentes biológicas exógenas.
Para obtener guias sobre la preparación de muestras de células, visite
https://www.aatbio.com/resources/guides/cell-sample-preparation.html
Imagenes
Figura 1. El número de células CHO-K1 se midió con el kit de ensayo de ATP de luminiscencia PhosphoWorks™ en una placa blanca de 96 pocillos utilizando un lector de placas NOVOstar (BMG Labtech). La señal de luminiscencia de las células CHO-K1 hasta 100 células por pocillo se controló durante un máximo de 2 horas (factor Z’ = 0,6). El tiempo integrado fue de 1 segundo.
Figura 2. La respuesta a la dosis de ATP se midió con el kit de ensayo PhosphoWorks Luminescence ATP. Se controlaron las concentraciones de ATP de 10 uM a 0,1 nM durante un máximo de 5 horas a intervalos de 1 segundo.
Productos Relacionados
| PhosphoWorks™ Luminometric ATP Assay Kit *Bright Glow* |
| PhosphoWorks™ Luminometric ATP Assay Kit *DTT-Free* |
Bibliografía
Coronary Endothelium No-Reflow Injury Is Associated with ROS-Modified Mitochondrial Fission through the JNK-Drp1 Signaling Pathway
Authors: Chen, Yi and Liu, Chen and Zhou, Peng and Li, Jiannan and Zhao, Xiaoxiao and Wang, Ying and Chen, Runzhen and Song, Li and Zhao, Hanjun and Yan, Hongbing
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High throughput cell-based assay for identification of glycolate oxidase inhibitors as a potential treatment for Primary Hyperoxaluria Type 1
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NT1014, a novel biguanide, inhibits ovarian cancer growth in vitro and in vivo
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The Different Effects of Atorvastatin and Pravastatin on Cell Death and PARP Activity in Pancreatic NIT-1 Cells
Authors: Chen, Ya-Hui and Chen, Yi-Chun and Liu, Chin-San and Hsieh, Ming-Chia
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Glutamine promotes ovarian cancer cell proliferation through the mTOR/S6 pathway
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BPA-induced DNA hypermethylation of the master mitochondrial gene PGC-1α contributes to cardiomyopathy in male rats
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Referencias
Ver todas las 108 referencias: Citation Explorer
Cell-surface-localized ATP detection with immobilized firefly luciferase
Authors: Nakamura M, Mie M, Funabashi H, Yamamoto K, Ando J, Kobatake E.
Journal: Anal Biochem (2006): 61
Ca2+ oscillations stimulate an ATP increase during fertilization of mouse eggs
Authors: Campbell K, Swann K.
Journal: Dev Biol (2006): 225
An efficient method for quantitative determination of cellular ATP synthetic activity
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Journal: J Biomol Screen (2006): 310
Basic evaluation for new antimicrobial susceptibility testing of Mycobacterium leprae by bioluminescence assay (ATP method)
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Journal: Nihon Hansenbyo Gakkai Zasshi (2006): 227
Cells die with increased cytosolic ATP during apoptosis: a bioluminescence study with intracellular luciferase
Authors: Zamaraeva MV, Sabirov RZ, Maeno E, Ando-Akatsuka Y, Bessonova SV, Okada Y.
Journal: Cell Death Differ (2005): 1390
Do rat cardiac myocytes release ATP on contraction
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Journal: Am J Physiol Cell Physiol (2005): C609
ATP bioluminescence assay for estimation of microbial populations of fresh-cut melon
Authors: Ukuku DO, Sapers GM, Fett WF.
Journal: J Food Prot (2005): 2427
Determination of ATP impurity in adenine dinucleotides
Authors: Pojoga LH, Haghiac ML, Moose JE, Hilderman RH.
Journal: Nucleosides Nucleotides Nucleic Acids (2004): 581
Identification of kinase inhibitors by an ATP depletion method
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ATP amplification for ultrasensitive bioluminescence assay: detection of a single bacterial cell
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Application Notes
Multicolor Analysis of Cell Proliferation Using a New Panel of CytoTell™ Fluorescent Dyes
Relative Brightness of Fluorescent Dyes
Abbreviation of Common Chemical Compounds Related to Peptides
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