Rhodamine B, hexyl ester, perchlorate

Rodamina B, éster hexílico, perclorato

Descripción

Los tintes de rodamina cargados positivamente (como los ésteres de rodamina y las rosaminas) se localizan selectivamente en las mitocondrias, por lo que se utilizan ampliamente para marcar mitocondrias de células vivas. Este éster de rodamina en particular tiñe las mitocondrias de naranja con fluorescencia. Sus propiedades espectrales son similares a las de TRITC, lo que hace que el uso de hexil éster de rodamina B sea bastante conveniente.

CatalogoProductoPresentación
AAT-22211Rhodamine B, hexyl ester, perchlorate10 mg

Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelar (< -15 °C); Minimizar la exposición a la luz.

Espectro

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Propiedades espectrales

Absorbancia (nm)541
Coeficiente de extinción (cm -1 M -1)1060001
Exitación546
Emisión568
Rendimiento cuántico0.312
1 Metanol, 2 Agua

Calculadora

Preparación de la solución de stock común
Volumen de DMSO necesario para reconstituir la masa específica de rodamina B, hexil éster, perclorato a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM159.446 µL797.232 µL1.594 mL7.972 mL15.945 mL
5 mM31.889 µL159.446 µL318.893 µL1.594 mL3.189 mL
10 mM15.945 µL79.723 µL159.446 µL797.232 µL1.594 mL

Imagen

Figura 1. Estructura química de Rhodamine B, hexyl ester, perchlorate (Rodamina B, éster hexílico, perclorato)

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Bibliografía

Mechanism of transdermal permeation promotion of lipophilic drugs by ethosomes
Authors: Yang, Li and Wu, Lifang and Wu, Dongze and Shi, Deshun and Wang, Tai and Zhu, Xiaoliang
Journal: International journal of nanomedicine (2017): 3357

Referencias

Ver todas las 29 referencias: Citation Explorer

Interaction of a mitochondrial membrane potential-sensitive dye, rhodamine 800, with rat mitochondria, cells, and perfused hearts
Authors: Jilkina O, Kong HJ, Hwi L, Kuzio B, Xiang B, Manley D, Jackson M, Kupriyanov VV.
Journal: J Biomed Opt (2006): 14009

Nature and significance of the electron-dense bodies of the endospores of rhinosporidium seeberi: their reactions with MTT (3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide) and TMRE (tetramethyl-rhodamine ethyl ester)
Authors: Arseculeratne SN, Atapattu DN, Wickramaratne K.
Journal: Med Mycol (2005): 261

Photophysical, photochemical, and tumor-selectivity properties of bromine derivatives of rhodamine-123
Authors: Lacerda SH, Abraham B, Stringfellow TC, Indig GL.
Journal: Photochem Photobiol (2005): 1430

Isolation and enrichment of murine spermatogonial stem cells using rhodamine 123 mitochondrial dye
Authors: Lo KC, Brugh VM, 3rd, Parker M, Lamb DJ.
Journal: Biol Reprod (2005): 767

Cadmium-induced mitochondrial membrane-potential dissipation does not necessarily require cytosolic oxidative stress: studies using rhodamine-123 fluorescence unquenching
Authors: Bolduc JS, Denizeau F, Jumarie C.
Journal: Toxicol Sci (2004): 299

Interference of calmidazolium with measurement of mitochondrial membrane potential using the tetraphenylphosphonium electrode or the fluorescent probe rhodamine 123
Authors: Saris NE, Teplova VV, Odinokova IV, Azarashvily TS.
Journal: Anal Biochem (2004): 109

Fluorescence lifetime imaging (FLIM) of rhodamine 123 in living cells
Authors: Schneckenburger H, Stock K, Lyttek M, Strauss WS, Sailer R.
Journal: Photochem Photobiol Sci (2004): 127

Rhodamine 123 as a probe of mitochondrial membrane potential: evaluation of proton flux through F(0) during ATP synthesis
Authors: Baracca A, Sgarbi G, Solaini G, Lenaz G.
Journal: Biochim Biophys Acta (2003): 137

Rhodamine B as a mitochondrial probe for measurement and monitoring of mitochondrial membrane potential in drug-sensitive and -resistant cells
Authors: Reungpatthanaphong P, Dechsupa S, Meesungnoen J, Loetchutinat C, Mankhetkorn S.
Journal: J Biochem Biophys Methods (2003): 1

Microfluorometric study of oxygen dependence of (1″-pyrene butyl)-2-rhodamine ester probe in mitochondria of living cells
Authors: Ribou AC, Vigo J, Kohen E, Salmon JM.
Journal: J Photochem Photobiol B (2003): 107

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