Sustrato de peroxidasa Azul Amplite® permite realizar ensayos de detección rápida de HRP con mayor sensibilidad que los sustratos colorimétricos de HRP (como TMB y OPD).
Descripción
Sustrato de peroxidasa Azul Amplite®
Hay una variedad de sustratos disponibles para detectar la actividad de la peroxidasa en los ensayos ELISA. Los sustratos colorimétricos de HRP (p. ej., TMB, OPD y ABTS) se han utilizado ampliamente durante años. Cada uno de estos sustratos varía mucho con respecto a sus características de rendimiento, como la sensibilidad de detección, el rango de trabajo y las relaciones señal/ruido alcanzables.
Nuestro sustrato de peroxidasa Amplite® Blue es fluorogénico soluble para la detección de la actividad de la peroxidasa. Amplite® Blue permite realizar ensayos de detección rápida de HRP con mayor sensibilidad que los sustratos colorimétricos de HRP (como TMB y OPD). El producto fluorescente de Amplite® Blue no fotoblanquea y exhibe una línea de base plana en los ensayos, lo que facilita la sensibilidad de detección de bajo nivel y permite altas relaciones señal-ruido.
Nombre en Ingles: Amplite® Blue peroxidase substrate
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-11005 | Sustrato de peroxidasa Azul Amplite® | 25 mg |
Importante, Solo para uso en investigación (RUO). Almacenamiento: Congelación a <-15 °C. Minimizar la exposición a la luz.
Plataforma
Lector de Microplacas de Flourescencia
| Excitación | 324 nm |
| Emisión | 409 nm |
| Cuttoff | 350 nm |
| Placa recomendada | Placa negra |
Notas importantes
Es importante almacenar a <-15 °C y debe almacenarse en un lugar fresco y oscuro.
Se puede utilizar en un plazo de 12 meses a partir de la fecha de recepción.
Imagen
Figura 1. La respuesta a la dosis de HRP se midió con el kit de ensayo de peroxidasa fluorimétrica Amplite® en una placa negra utilizando un lector de microplacas de fluorescencia Gemini (Molecular Devices).
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Referencias
Ver todas las 61 referencias: Citation Explorer
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Authors: Rajashekhara E, Hosoda A, Sode K, Ikenaga H, Watanabe K.
Journal: Biotechnol Prog (2006): 334
Galactose oxidase action on galactose containing glycolipids–a fluorescence method
Authors: Fortelius M, Mattjus P.
Journal: Chem Phys Lipids (2006): 103
Effects of chronic ethanol administration on the activities and relative synthetic rates of myelin and synaptosomal plasma membrane-associated sialidase in the rat brain
Authors: Azuine MA, Patel SJ, Lakshman MR.
Journal: Neurochem Int (2006): 67
Mitochondrial reactive oxygen species in mice lacking superoxide dismutase 2: attenuation via antioxidant treatment
Authors: Morten KJ, Ackrell BA, Melov S.
Journal: J Biol Chem (2006): 3354
A new, sensitive method for enzyme kinetic studies of scarce glucosides
Authors: Mazura P, Fohlerova R, Brzobohaty B, Kiran NS, J and a L., undefined
Journal: J Biochem Biophys Methods (2006): 55
Evaluation of assays for the measurement of bovine neutrophil reactive oxygen species
Authors: Rinaldi M, Moroni P, Paape MJ, Bannerman DD.
Journal: Vet Immunol Immunopathol. (2006)
Kinetic analysis of semisynthetic peroxidase enzymes containing a covalent DNA-heme adduct as the cofactor
Authors: Fruk L, Muller J, Niemeyer CM.
Journal: Chemistry (2006): 7448
K+-independent actions of diazoxide question the role of inner membrane KATP channels in mitochondrial cytoprotective signaling
Authors: Drose S, Br and t U, Hanley PJ.
Journal: J Biol Chem (2006): 23733
A Role for Mitochondrial Dysfunction in Perpetuating Radiation-Induced Genomic Instability
Authors: Kim GJ, Fiskum GM, Morgan WF.
Journal: Cancer Res (2006): 10377
Sexual Dimorphism in Oxidant Status in Spontaneously Hypertensive Rats
Authors: Sullivan JC, Sasser JM, Pollock JS.
Journal: Am J Physiol Regul Integr Comp Physiol. (2006)
Application notes
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