Calbryte™ 590 es una nueva generación de indicadores fluorescentes rojos para la medición del calcio intracelular.
Descripción
El ensayo de flujo de calcio intracelular es un método ampliamente utilizado en el monitoreo de las vías de transducción de señales y el cribado de alto rendimiento de los receptores acoplados a proteínas G (GPCR) y los objetivos de los canales de calcio. Seguidos por la introducción de Rhod-2 en 1989, Rhod-4 y Cal-590 se desarrollaron más tarde con una relación señal / fondo mejorada, y se convirtieron en los indicadores fluorescentes rojos de Ca2 + ampliamente utilizados para microscopía confocal, citometría de flujo y aplicaciones de detección de alto rendimiento.
Calbryte™ 590 es una nueva generación de indicadores fluorescentes rojos para la medición del calcio intracelular. Su relación señal/fondo muy mejorada y sus propiedades de retención intracelular hacen de Calbryte™ 590 el indicador fluorescente rojo más robusto para evaluar GPCR y objetivos de canales de calcio, así como para detectar sus agonistas y antagonistas en células vivas.
Catalogo | Producto | Presentación |
---|---|---|
AAT-20706 | Calbryte™ 590, potassium salt | 5 x 50 ug |
AAT-20707 | Calbryte™ 590, potassium salt | 1 mg |
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelar a (< -15 °C), Minimizar la exposición a la luz.
Espectro
Abrir en Advanced Spectrum Viewer
![](http://cidsamexico.com/wp-content/uploads/2023/01/image-77.png)
Propiedades espectrales
Excitación (nm) | 581 |
Emisión (nm) | 593 |
Calculadora
Preparación de la solución de stock común
Volumen de agua necesario para reconstituir la masa específica de Calbryte™ 590, sal de potasio a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para obtener los buffers experimentales/fisiológicos apropiados.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 93.025 µL | 465.125 µL | 930.25 µL | 4.651 mL | 9.302 mL |
5 mM | 18.605 µL | 93.025 µL | 186.05 µL | 930.25 µL | 1.86 mL |
10 mM | 9.302 µL | 46.512 µL | 93.025 µL | 465.125 µL | 930.25 µL |
Imagen
![](http://cidsamexico.com/wp-content/uploads/2023/03/image-1.png)
Figura 1. Espectros de emisión de fluorescencia de Calbryte™ 590 en solución que contiene 0 a 39 μM libre Ca2+.
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Referencias
Ver todas las 53 referencias: Citation Explorer
A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux
Authors: Bailey S, Macardle PJ.
Journal: J Immunol Methods (2006): 220
Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy
Authors: Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.
Journal: Gen Physiol Biophys (2004): 357
Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry
Authors: Patel H, Porter RH, Palmer AM, Croucher MJ.
Journal: Br J Pharmacol (2003): 671
Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics
Authors: Loughrey CM, MacEachern KE, Cooper J, Smith GL.
Journal: Cell Calcium (2003): 1
A sensitive method for the detection of foot and mouth disease virus by in situ hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification
Authors: Zhang Z, Kitching P.
Journal: J Virol Methods (2000): 187
Kinetics of onset of mouse sperm acrosome reaction induced by solubilized zona pellucida: fluorimetric determination of loss of pH gradient between acrosomal lumen and medium monitored by dapoxyl (2-aminoethyl) sulfonamide and of intracellular Ca(2+) chang
Authors: Rockwell PL, Storey BT.
Journal: Mol Reprod Dev (2000): 335
MRP2, a human conjugate export pump, is present and transports fluo 3 into apical vacuoles of Hep G2 cells
Authors: Cantz T, Nies AT, Brom M, Hofmann AF, Keppler D.
Journal: Am J Physiol Gastrointest Liver Physiol (2000): G522
Use of co-loaded Fluo-3 and Fura Red fluorescent indicators for studying the cytosolic Ca(2+)concentrations distribution in living plant tissue
Authors: Walczysko P, Wagner E, Albrechtova JT.
Journal: Cell Calcium (2000): 23
[Ca2+]i following extrasystoles in guinea-pig trabeculae microinjected with fluo-3 – a comparison with frog skeletal muscle fibres
Authors: Wohlfart B., undefined
Journal: Acta Physiol Scand (2000): 1
Determination of the intracellular dissociation constant, K(D), of the fluo-3. Ca(2+) complex in mouse sperm for use in estimating intracellular Ca(2+) concentrations
Authors: Rockwell PL, Storey BT.
Journal: Mol Reprod Dev (1999): 418
Aplication Notes (en Ingles)
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