iFluor® 450 SE proporciona una herramienta conveniente para marcar anticuerpos monoclonales, policlonales u otras proteínas (>10 kDa) para aplicaciones de imágenes de fluorescencia y citometría de flujo.
Descripción
Los tintes iFluor® de AAT Bioquest están optimizados para marcar proteínas, en particular anticuerpos. Estos tintes son brillantes, fotoestables y tienen un enfriamiento mínimo de las proteínas. Pueden excitarse bien con las principales líneas láser de los instrumentos de fluorescencia (p. ej., 350, 405, 488, 555 y 633 nm).
Los colorantes iFluor® 450 tienen un máximo de excitación y emisión de fluorescencia de ~451 nm y ~502 nm respectivamente. Estas características espectrales los convierten en una excelente opción para aplicaciones multicolores. iFluor® 450 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de proteínas.
iFluor® 450 SE proporciona una herramienta conveniente para marcar anticuerpos monoclonales, policlonales u otras proteínas (>10 kDa) para aplicaciones de imágenes de fluorescencia y citometría de flujo. Los conjugados de tinte iFluor® 450 son brillantes y brindan un color adicional entre Alexa Fluor® 430 y 488 (ThermoFisher).
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-1026 | iFluor® 450 succinimidyl ester | 1mg |
| AAT-71026 | iFluor® 450 succinimidyl ester | 100 ug |
| AAT-71506 | iFluor® 450 succinimidyl ester | 5mg |
| AAT-71556 | iFluor® 450 succinimidyl ester | 10mg |
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelación (< -15 °C). Minimizar la exposición a la luz.
Propiedades fisicas
| Peso Molecular | 603.69 |
| Disolvente | DMSO |
Espectro
Abrir en Advanced Spectrum Viewer
Propiedades espectrales
| Factor de corrección (260 nm) | 0.45 |
| Factor de corrección (280 nm) | 0.27 |
| Coeficiente de extinción (cm -1 M -1) | 400001 |
| Excitación (nm) | 451 |
| Emisión (nm) | 502 |
| Rendimiento cuántico | 0.821 |
Calculadora
Preparación de la solución de stock común
Volumen de DMSO necesario para reconstituir la masa específica de succinimidil éster iFluor® 450 a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de la muestra para conocer los buffers experimentales/fisiológicos apropiados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 165.648 µL | 828.24 µL | 1.656 mL | 8.282 mL | 16.565 mL |
| 5 mM | 33.13 µL | 165.648 µL | 331.296 µL | 1.656 mL | 3.313 mL |
| 10 mM | 16.565 µL | 82.824 µL | 165.648 µL | 828.24 µL | 1.656 mL |
Imagenes
Figura 1. Las células HL-60 se incubaron con (Rojo, +) o sin (Verde, -) HLA-ABC antihumano (W6/32 mAb), seguido de conjugado de IgG anti-ratón de cabra iFluor® 450. La señal de fluorescencia se controló utilizando un citómetro de flujo ACEA NovoCyte en el canal FITC.
Productos Similares
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| iFluor® 350 succinimidyl ester | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
| iFluor® 488 succinimidyl ester | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
| iFluor® 514 succinimidyl ester | 511 | 527 | 750001 | 0.831 | 0.265 | 0.116 |
| iFluor® 532 succinimidyl ester | 537 | 560 | 900001 | 0.681 | 0.26 | 0.16 |
| iFluor® 555 succinimidyl ester | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 594 succinimidyl ester | 588 | 604 | 1800001 | 0.531 | 0.05 | 0.04 |
| iFluor® 633 succinimidyl ester | 640 | 654 | 2500001 | 0.291 | 0.062 | 0.044 |
| iFluor® 647 succinimidyl ester | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
| iFluor® 660 succinimidyl ester | 663 | 678 | 2500001 | 0.261 | 0.07 | 0.08 |
Bibliografía
Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: Metabolomics (Los Angel) (2017): 2153–0769
Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: Proceedings of the National Academy of Sciences (2016): E2011–E2018
Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: International Journal of Cancer (2012): E425–E436
Referencias
Ver todas las 49 referencias: Citation Explorer
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Journal: Mol Biosyst (2011): 1766
Visualizing dengue virus through Alexa Fluor labeling
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Journal: Biochem Biophys Res Commun (2011): 211
Neuroanatomical basis of clinical joint application of “Jinggu” (BL 64, a source-acupoint) and “Dazhong” (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
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Journal: Zhen Ci Yan Jiu (2011): 262
Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119
Alexa Fluor 546-ArIB[V11L;V16A] is a potent ligand for selectively labeling alpha 7 nicotinic acetylcholine receptors
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Journal: J Neurochem (2010): 994
Asymmetric trimethine 3H-indocyanine dyes: efficient synthesis and protein labeling
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Journal: Org Biomol Chem (2010): 4249
Neuroanatomical characteristics of acupoint “Chengshan” (BL 57) in the rat: a cholera toxin subunit B conjugated with Alexa Fluor 488 method study
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Journal: Zhen Ci Yan Jiu (2010): 433
Photoactivatable and photoconvertible fluorescent probes for protein labeling
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Novel Alexa Fluor-488 labeled antagonist of the A(2A) adenosine receptor: Application to a fluorescence polarization-based receptor binding assay
Authors: Kecskes M, Kumar TS, Yoo L, Gao ZG, Jacobson KA.
Journal: Biochem Pharmacol (2010): 506
Application Notes
iFluor® Dye Selection Guide
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
FAQ
What are the spectral properties of iFluor dyes?
Are any of the cyanine dyes infrared?
Are coumarin dyes pH sensitive?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
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