Indicador de calcio Calbryte™-520L AM. Calbryte 520L tiene una baja afinidad con los iones de calcio con un Kd ∼91 μM.
Descripción
Indicador Calbryte™-520L, AM
El ensayo de flujo de calcio intracelular es un método ampliamente utilizado para monitorear vías de transducción de señales y detección de alto rendimiento de receptores acoplados a proteínas G (GPCR) y objetivos de canales de calcio.
Luego de la introducción de Fluo-3 en 1989, Fluo-4, Fluo-8 y Cal-520 se desarrollaron posteriormente con relaciones señal-fondo mejoradas y pronto se convirtieron en los indicadores de Ca2+ de elección para microscopía confocal, citometría de flujo y alta aplicaciones de detección de rendimiento. Sin embargo, todavía hay algunas advertencias con Fluo-4. Por ejemplo, al igual que el Fluo-3, el Fluo-4 presenta una retención intracelular deficiente y se requiere el uso de probenecid para evitar que el Fluo-4 cargado en las células se escape de las células.
El uso de probenecid con ensayos de calcio basados en Fluo-4 compromete los resultados del ensayo ya que está bien documentado que probenecid tiene una variedad de efectos celulares complicados. Calbryte 520L, AM es un nuevo indicador de calcio fluorescente y permeable a las células. Al igual que otras cargas de células AM de tinte, el éster AM de Calbryte 520L no es fluorescente y, una vez que ingresa a la célula, es hidrolizado por la esterasa intracelular y se activa.
El indicador activado es una molécula polar que ya no es capaz de difundirse libremente a través de la membrana celular y queda esencialmente atrapada dentro de las células. Calbryte 520L tiene una baja afinidad por los iones de calcio con una Kd ∼ 91 µM. Calbryte 520L produce una señal de fluorescencia brillante en presencia de calcio en altas concentraciones. Tiene la misma longitud de onda de excitación y emisión que Fluo-4; por lo tanto, los mismos ajustes de ensayo de Fluo-4 se pueden aplicar fácilmente a los ensayos de calcio basados en Calbryte 520L.
Nombre en ingles: Calbryte™-520L AM
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelar a (< -15 °C), Minimizar la exposición a la luz.
Catalogo | Producto | Presentación |
---|---|---|
AAT-20640 | Indicador Calbryte™-520L AM | 10 x 50 ug |
Plataforma
Microscopio de Fluorescencia
Excitación | FITC |
Emisión | FITC |
Placa Recomendada | Pared negra/Fondo claro |
Lector de microplacas de Fluorescencia
Excitación | 490 |
Emisión | 525 |
Cutoff | 515 |
Placa Recomendada | Pared Negra / fondo claro |
Especificaciones Instrumento | Modo de lectura inferior / manipulación de liquidos programable |
Espectro
Abrir en Advanced Spectrum Viewer
![](https://cidsamexico.com/wp-content/uploads/2023/08/image-3.png)
Propiedades espectrales
Excitación (nm) | 493 |
Emisión (nm) | 515 |
Rendimiento cuántico | 0.751 |
Calculadora
Volumen de DMSO necesario para reconstituir la masa específica de Calbryte™-520L AM a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 114.847 µL | 574.237 µL | 1.148 mL | 5.742 mL | 11.485 mL |
5 mM | 22.969 µL | 114.847 µL | 229.695 µL | 1.148 mL | 2.297 mL |
10 mM | 11.485 µL | 57.424 µL | 114.847 µL | 574.237 µL | 1.148 mL |
Imagen
![](https://cidsamexico.com/wp-content/uploads/2023/08/image-4.png)
Figura 1. Ca2+ Dosis-respuesta dependiente de Calbryte 520L
Productos Relacionados
Name | Excitation (nm) | Emission (nm) | Quantum yield |
Calbryte™ 520, potassium salt | 493 | 515 | 0.751 |
Calbryte™ 590, potassium salt | 581 | 593 | – |
Calbryte™ 630, potassium salt | 607 | 624 | – |
Calbryte™-520XL, potassium salt | 493 | 515 | 0.751 |
Bibliografía
Calreticulin regulates TGF-β1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling
Authors: Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling
Journal: The International Journal of Biochemistry & Cell Biology (2017)
Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells
Authors: Lu, Jiang and Yao, Xue-qin and Luo, Xin and Wang, Yu and Chung, Sookja Kim and Tang, He-xin and Cheung, Chi Wai and Wang, Xian-yu and Meng, Chen and Li, Qing and others, undefined
Journal: Neural Regeneration Research (2017): 945
Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels
Authors: Yang, Gang and Xiao, Zhenghua and Ren, Xiaomei and Long, Haiyan and Ma, Kunlong and Qian, Hong and Guo, Yingqiang
Journal: Scientific Reports (2017): 41781
Dexmedetomidine reduces hypoxia/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons
Authors: Liu, Jia and Du, Qing and Zhu, He and Li, Yu and Liu, Maodong and Yu, Shoushui and Wang, Shilei
Journal: Int J Clin Exp Med (2017): 6861–6868
Di (2-ethylhexyl) phthalate-induced apoptosis in rat INS-1 cells is dependent on activation of endoplasmic reticulum stress and suppression of antioxidant protection
Authors: Sun, Xia and Lin, Yi and Huang, Qiansheng and Shi, Junpeng and Qiu, Ling and Kang, Mei and Chen, Yajie and Fang, Chao and Ye, Ting and Dong, Sijun
Journal: Journal of cellular and molecular medicine (2015): 581–594
The effect of mitochondrial calcium uniporter on mitochondrial fission in hippocampus cells ischemia/reperfusion injury
Authors: Zhao, Lantao and Li, Shuhong and Wang, Shilei and Yu, Ning and Liu, Jia
Journal: Biochemical and biophysical research communications (2015): 537–542
Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living rats
Authors: Liang, Nan and Wang, Peng and Wang, Shilei and Li, Shuhong and Li, Yu and Wang, Jinying and Wang, Min
Journal: Journal of Neural Transmission (2014): 593–600
Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells
Authors: Li, Qing and Lu, Jiang and Wang, Xianyu and others, undefined
Journal: Neural regeneration research (2014): 2002
Fungus induces the release of IL-8 in human corneal epithelial cells, via Dectin-1-mediated protein kinase C pathways.
Authors: Peng, Xu-Dong and Zhao, Gui-Qiu and Lin, Jing and Jiang, Nan and Xu, Qiang and Zhu, Cheng-Cheng and Qu, Jain-Qiu and Cong, Lin and Li, Hui
Journal: International journal of ophthalmology (2014): 441–447
Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve–mast cell interaction in a hapten-induced mouse model of atopic dermatitis
Authors: Hagiyama, M and Inoue, T and Furuno, T and Iino, T and Itami, S and Nakanishi, M and Asada, H and Hosokawa, Y and Ito, A
Journal: British Journal of Dermatology (2013): 771–778
Referencias
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Aplication Notes
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
FAQ
Are there any calcium indicators that don’t require probenecid (PBC)?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
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AssayWise
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