Proteina Bacteriorhodopsin (Hs)

Descripción

La bacteriorrodopsina (BR) es una proteína transmembrana 7 que actúa como una bomba de protones impulsada por la luz en las arqueobacterias. Debido a su ligando coloreado unido covalentemente, BR se usa ampliamente como proteína de membrana de control, por ejemplo, para ensayos de cristalización y experimentos bioquímicos/biofísicos. Proporcionamos BR de alta pureza, preparados como parches de membrana púrpura de Halobacterium salinarum.

Catalogo	Producto	Presentación
CU-28903	Proteina Bacteriorhodopsin (Hs)	5 x 100 ug

Caracteristicas

Alternative names	Bacteriorhodopsin, BR, HsBR, Bacteriopsin
UniProt Number	P02945
Protein class	7-transmembrane protein
Organism	Halobacterium salinarum S9
Sequence, explained	Full-length, wildtype sequence with N-terminal signal sequence which is mostly absent in the preparation
Sequence, One-Letter Code	MLELLPTAVE GVSQAQITGR PEWIWLALGT ALMGLGTLYF LVKGMGVSDP DAKKFYAITT LVPAIAFTMY LSMLLGYGLT MVPFGGEQNP IYWARYADWL FTTPLLLLDL ALLVDADQGT ILALVGADGI MIGTGLVGAL TKVYSYRFVW WAISTAAMLY ILYVLFFGFT SKAESMRPEV ASTFKVLRNV TVVLWSAYPV VWLIGSEGAG IVPLNIETLL FMVLDVSAKV GFGLILLRSR AIFGEAEAPE PSAGDGAAAT SD
Affinity tags	None
Expression Host	Halobacterium salinarum S9
Size (excluding additional elements)	262 amino acids; 28,256 Da
Buffer composition	Purple membrane (PM) patches in H2O, 0.02% NaN3
Purity	>95%, determined via SDS-PAGE
Purified via	Sequential centrifugation
Absorbance	Extinction coefficient at 560 nm: 63,000 M-1 cm-1.
Activity	Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 560 nm = 2.3
Function	Photoreceptor protein undergoing a photocycle, Retinal protein, light-driven proton pump
Literature references	Kubicek, Jan, et al. “Controlled in meso phase crystallization–a method for the structural investigation of membrane proteins.” PloS one 7.4 (2012): e35458.Zscherp, Christian, Ramona Schlesinger, and Joachim Heberle. “Time-resolved FT-IR spectroscopic investigation of the pH-dependent proton transfer reactions in the E194Q mutant of bacteriorhodopsin.” Biochemical and biophysical research communications 283.1 (2001): 57-63.

Preparación
Expression: system Halobacterium salinarum S9
Purification: Differential centrifugation
Buffer: Purple membrane (PM) patches in H2O, 0.02% NaN3
Concentration: 8.3 mg/mL
Volume: 65 µL per 100 µg aliquot

Control de Calidad
Purity: : >98%, as determined by SDS-PAGE, See Fig. 1
Activity: Binding of ligand all-trans retinal, covalently bound to a lysine residue.
Evaluation of UV-VIS spectrum: absorbance ratio 280 nm / 560 nm = 2.3, (see Figure 2)

Aplicaciones
• SDS-PAGE
• Western Blot
• Protein Crystallization
• Biochemical and biophysical analyses

Envio y Almacenamiento

Este producto se envia en hielo seco y se almacena a -80°C. Importante: la bacteriorrodopsina es sensible a la luz y debe almacenarse en la oscuridad.

Imagenes

Descargo de responsabilidad: Nuestros productos están destinados para investigacion en aplicaciones de biología molecular. Estos productos no están destinados al diagnóstico, prevención o tratamiento de una enfermedad.