Este reactivo con amina succinimidil éster Texas Red®-X, es un reemplazo superior al Texas Red® (marca registrada de Invitrogen).
Descripción
Este reactivo con amina succinimidil éster Texas Red®-X, es un reemplazo superior al Texas Red (Texas Red® es la marca registrada de Invitrogen). Tiene las mismas propiedades espectrales que Texas Red, pero es mucho más fácil de conjugar con una biomolécula con un rendimiento de conjugación mucho mayor. Se puede utilizar para crear bioconjugados fluorescentes de color rojo brillante con un máximo de excitación/emisión de ~595/615 nm. En comparación con Texas Red®, este colorante reactivo contiene un espaciador de aminohexanoilo (“X”) adicional de siete átomos entre el fluoróforo y el grupo éster de succinimidilo. Este espaciador ayuda a separar el fluoróforo de su punto de unión, reduciendo potencialmente la interacción del fluoróforo con la biomolécula a la que está conjugado. En comparación con los isómeros mixtos (#474), este producto es el isómero 5 único, lo que brinda resultados más reproducibles.
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-474 | Texas Red-X, succinimidyl ester *Single isomer* *178623-11-5* | 5mg |
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelación (< -15 °C). Minimizar la exposición a la luz.
Propiedades fisicas
| Peso Molecular | 816.94 |
| Disolvente | DMSO |
Espectro
Abrir en Advanced Spectrum Viewer
Propiedades espectrales
| Factor de corrección (260 nm) | 0.23 |
| Factor de corrección (280 nm) | 0.36 |
| Factor de corrección (565 nm) | 0.4472 |
| Coeficiente de extinción (cm -1 M -1) | 1160001 |
| Excitación (nm) | 586 |
| Emisión (nm) | 603 |
| Rendimiento cuántico | 0.932, 0.351 |
Calculadora
Preparación de la solución de stock común
Volumen de DMSO necesario para reconstituir la masa específica de Texas Red-X, succinimidil éster Isómero único 178623-11-5 a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 122.408 µL | 612.04 µL | 1.224 mL | 6.12 mL | 12.241 mL |
| 5 mM | 24.482 µL | 122.408 µL | 244.816 µL | 1.224 mL | 2.448 mL |
| 10 mM | 12.241 µL | 61.204 µL | 122.408 µL | 612.04 µL | 1.224 mL |
Imagenes
Figura 1. Estructura quimica de Texas Red-X, succinimidyl ester *Single isomer* *CAS 178623-11-5*
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Referencias
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Application Notes (en Ingles)
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)