iFluor® 350 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de proteínas.
Descripción
Los tintes iFluor® de AAT Bioquest están optimizados para marcar proteínas, en particular, anticuerpos. Estos tintes son brillantes, fotoestables y tienen un enfriamiento mínimo de las proteínas. Pueden excitarse bien con las principales líneas láser de los instrumentos de fluorescencia (ejem. 350, 405, 488, 555 y 633 nm).
Los tintes iFluor® 405 tienen un máximo de excitación y emisión de fluorescencia de ~406 nm y ~427 nm respectivamente. Estas características espectrales los convierten en una excelente alternativa al colorante de etiquetado AMCA y Alexa Fluor® 405 (Alexa Fluor® es la marca registrada de Invitrogen).
iFluor® 405 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de proteínas.
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-1021 | iFluor® 405 succinimidyl ester | 1mg |
| AAT-71021 | iFluor® 405 succinimidyl ester | 100 ug |
| AAT-71501 | iFluor® 405 succinimidyl ester | 5mg |
| AAT-71551 | iFluor® 405 succinimidyl ester | 10mg |
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelación (< -15 °C). Minimizar la exposición a la luz.
Propiedades fisicas
| Peso Molecular | 755.58 |
| Disolvente | DMSO |
Espectro
Abrir en Advanced Spectrum Viewer
Propiedades espectrales
| Factor de corrección (260 nm) | 0.48 |
| Factor de corrección (280 nm) | 0.77 |
| Coeficiente de extinción (cm -1 M -1) | 370001 |
| Excitación (nm) | 403 |
| Emisión (nm) | 427 |
| Rendimiento cuántico | 0.911 |
Calculadora
Preparación de la solución de stock común
Volumen de DMSO necesario para reconstituir la masa específica de succinimidil éster iFluor® 405 a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de la muestra para conocer los buffers experimentales/fisiológicos apropiados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 132.349 µL | 661.743 µL | 1.323 mL | 6.617 mL | 13.235 mL |
| 5 mM | 26.47 µL | 132.349 µL | 264.697 µL | 1.323 mL | 2.647 mL |
| 10 mM | 13.235 µL | 66.174 µL | 132.349 µL | 661.743 µL | 1.323 mL |
Imagenes
Figura 1. Las células HL-60 se incubaron con (rojo, +) o sin (verde, -) anti-HLA-ABC humano (W6/32 mAb), seguido de iFluor® 405 cabra anti-ratón IgG (H&L). La señal de fluorescencia se controló utilizando un citómetro de flujo ACEA NovoCyte en el canal Pacific Blue (Ex/Em=405/445 nm).
Productos Similares
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| iFluor® 350 succinimidyl ester | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
| iFluor® 488 succinimidyl ester | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
| iFluor® 514 succinimidyl ester | 511 | 527 | 750001 | 0.831 | 0.265 | 0.116 |
| iFluor® 532 succinimidyl ester | 537 | 560 | 900001 | 0.681 | 0.26 | 0.16 |
| iFluor® 555 succinimidyl ester | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 594 succinimidyl ester | 588 | 604 | 1800001 | 0.531 | 0.05 | 0.04 |
| iFluor® 633 succinimidyl ester | 640 | 654 | 2500001 | 0.291 | 0.062 | 0.044 |
| iFluor® 647 succinimidyl ester | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
| iFluor® 660 succinimidyl ester | 663 | 678 | 2500001 | 0.261 | 0.07 | 0.08 |
Bibliografía
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Referencias
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Application Notes
iFluor® Dye Selection Guide
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
FAQ
What are common laser lines used in flow cytometry?
What are the spectral properties of iFluor dyes?
Are any of the cyanine dyes infrared?
Are coumarin dyes pH sensitive?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
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